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A method for the determination of protein and amino acid requirements of growing pigs under the practical feeding condition



A method for the determination of protein and amino acid requirements of growing pigs under the practical feeding condition



Japanese Journal of Zootechnical Science 56(8): 628-633



Two experiments were conducted to evaluate the use of the potassium indicator method using urinary nitrogen and potassium excretion rates for the determination of the protein and amino acid requirements of growing pigs kept in the practical ad libitum feeding condition. The formula used for calculating the retained N (%) is 100-(Kab/Nab) .times. (Nu/Ku) .times. R, in which Kab and Nab are, respectively, the digestable K content and the digestible N content in the diet, calculated by multiplying each concentration in the diet by each digestibility estimated by the Cr2O3 indicator method. Nu and Ku are the concentrations of N and K, respectively, in the urine. The recovery of absorbed K in the urine is shown as R, which is estimated by the relationship between retained K and gain in weight. The first experiment studied the effect of urinary collection time on the N/K concentration ratio with twelve female pigs weighing approximately 50 kg. The pigs were fed a practical diet supplemented with potassium (crude protein 12.7% and potassium 0.99%): urine was collected by introducing a catheter into the bladder at six collection periods (14:00, 18:00, 22:00, 6:00, 10:00 and 14:00). In the second experiment, twelve female pigs weighing approximately 53 kg were fed rice-soybean meal diets containing from 10 to 19% crude protein to estimate the protein and amino acid requirements by the postassium indicator method. Urine was collected during 14:00-15:00 daily throughout a three-day collection period. The results obtained were as follows. (1) There was no significant difference (P < 0.05) in the urinary N/K concentration rations among the six collection periods, though there was a tendency that ratio sampled during the night was higher than that sampled during the daytime. The result indicates that the time of urinary collection is not critical for the estimation of the retained nitrogen by the potassium indicator method with pigs fed ad libitum. (2) Nitrogen retention (g/day) was increased linearly as dietary crude protein was increased to 16%, and further increase in dietary crude protein was without effect. Using nitrogen retention as the response criterion, the calculated requirements for dietary crude protein and lysine were 15.2% and 0.76%, respectively.

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