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An assessment of the potential of peptidoglutaminase II in modifying the charge characteristics of proteins

Biochemical Society Transactions 13(2): 498
An assessment of the potential of peptidoglutaminase II in modifying the charge characteristics of proteins
Peptidoglutaminase II (PGase II) from Bacillus circulans deamidates the gamma -carboxyamide group of peptide-bound glutamine residues. Partially-purified PGase II was inactive against whey proteins or whey proteins subjected to a range of denaturing conditions. However, when whey proteins and casein were subjected to enzymic hydrolysis, incubation with PGase II liberated ammonia. Maximum PGase II activity was detected after 3-4% hydrolysis.

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Accession: 001297565

DOI: 10.1042/bst0130498

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