+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

CI628 inhibits follicle-stimulating hormone (FSH)-induced increases in FSH receptors of the rat ovary: requirement of estradiol for FSH action



CI628 inhibits follicle-stimulating hormone (FSH)-induced increases in FSH receptors of the rat ovary: requirement of estradiol for FSH action



Endocrinology 116(2): 715-722



Although estradiol (E2) alone does not increase receptors for FSH in granulosa cells, E2 priming before administration of FSH increases numbers of FSH receptors significantly compared with FSH alone. We hypothesized that if E2 is required for FSH to increase its own receptor, blocking estrogen action should prevent FSH-induced increases in FSH receptors. Five groups of hypophysectomized rats were injected sc with: saline at 0 h; the antiestrogen CI628 (1 mg) at -6 h; human FSH (hFSH, 2 micrograms) at 0 h; CI628 at -6 h, then hFSH at 0 h; and CI628 plus E2 (2 mg) at -6 h, then FSH at 0 h. Animals were decapitated at 0, 6, 12, or 24 h, and granulosa membrane receptors for FSH, LH, and nuclear receptors for E2 were measured. LH receptor levels increased only after administration of E2 before hFSH. Treatment with hFSH for 6 h increased numbers of FSH receptors 3-fold (P less than 0.01) without any increase in numbers of E2 receptors. At 12 and 24 h, hFSH increased numbers of FSH and E2 receptors 6- and 7-fold (P less than 0.01) over controls. CI628 prevented the hFSH-induced increases in FSH receptors at 6, 12, and 24 h. Administration of E2 concomitant with CI628 before hFSH significantly reversed the inhibitory effects of CI628 on hFSH-induced increases in FSH receptors. There were no changes in affinity of FSH or E2 receptors from 0 to 24 h. To determine whether E2 was acting on the adenylate cyclase system, the ability of hFSH to increase the content of cAMP in granulosa cells in each treatment group was determined. After an iv injection of hFSH, cAMP levels were similar in CI628- and saline-treated rats but had increased 6-fold (P less than 0.01) in hFSH or CI628 plus hFSH-treated animals. Thus, blocking hFSH-induced increases in FSH and E2 receptor appeared to have no effect on FSH stimulation of cAMP. In conclusion E2 appears to be required for FSH action, perhaps by acting within granulosa cells distal to the cAMP-adenylate cyclase system.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001311241

Download citation: RISBibTeXText

PMID: 2981676

DOI: 10.1210/endo-116-2-715


Related references

Metformin inhibits follicle-stimulating hormone (FSH) action in human granulosa cells: relevance to polycystic ovary syndrome. Journal of Clinical Endocrinology and Metabolism 98(9): E1491-E1500, 2013

Follicle stimulating hormone induced and estradiol induced restoration of luteinizing hormone human chorionic gonadotropin receptors in mono layer cultures of porcine granulosa cells. Biology of Reproduction 20(SUPPL 1): 12A, 1979

Antimüllerian hormone inhibits follicle-stimulating hormone-induced adenylyl cyclase activation, aromatase expression, and estradiol production in human granulosa-lutein cells. Fertility and Sterility 100(2): 585-92.E1, 2013

Spatiotemporal expression of epidermal growth factor receptor messenger RNA and protein in the hamster ovary: follicle stage-specific differential modulation by follicle-stimulating hormone, luteinizing hormone, estradiol, and progesterone. Biology of Reproduction 67(5): 1593-1604, 2002

Effects of exogenous luteinizing hormone or follicle stimulating hormone on endogenous follicle stimulating hormone progesterone and estradiol secretion. Biology of Reproduction 18(5): 820-828, 1978

Acute effects of estradiol and follicle stimulating hormone on specific binding of human iodine 125 iodo follicle stimulating hormone to rat ovarian granulosa cells in vivo and in vitro. Endocrinology 102(3): 876-883, 1978

Effect of cryptorchidism in the ram: changes in the concentrations of testosterone and estradiol and receptors for LH luteinizing hormone and FSH follicle-stimulating hormone. 1982

Expression of follicle stimulating hormone and luteinizing hormone receptors during follicular growth in the domestic cat ovary. Molecular Reproduction and Development 74(8): 989-996, 2007

Mechanism of action of luteinizing hormone and follicle-stimulating hormone in the ovary in vitro. Metabolism 26(4): 413-468, 1976

Mechanism of action of luteinizing hormone and follicle-stimulating hormone on the ovary in vitro. Metabolism: Clinical and Experimental 26(4): 413-468, 1977

Luteinizing hormone increases estradiol secretion but has no effect on progesterone concentrations in the late follicular phase of in vitro fertilization cycles in women treated with gonadotropin-releasing hormone agonist and follicle-stimulating hormone. Fertility & Sterility. 69(3): 450-453, Ch, 1998

Pro estral modifications of the plasma rate of luteinizing hormone and follicle stimulating hormone in rats exposed to the action of follicle stimulating hormone at the beginning of a 4 day cycle. Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences Serie D Sciences Naturelles 283(16): 1771-1773, 1976

Relationship of cytosol estradiol receptors of the hypothalamus and anterior pituitary to follicle stimulating hormone luteinizing hormone secretion in the female rat. Endocrinology 96(SUPPL): 305, 1975

The bi phasic pattern in the feedback action of ethinyl estradiol on the release of pituitary follicle stimulating hormone and luteinizing hormone. Journal Of Clinical Endocrinology & Metabolism: 882-887, 1971

Effects of follicle-stimulating hormone with and without luteinizing hormone on serum hormone concentrations, follicle growth, and intrafollicular estradiol and aromatase activity in gonadotropin-releasing hormone-immunized heifers. Biology of Reproduction 64(1): 368-374, 2001