Cadmium-induced ultrastructural changes in suspension-cultured tobacco cells (Nicotiana tabacum L. var xanthi)

Reese, R.N.; McCall, R.D.; Roberts, L.W.

Environmental and Experimental Botany 26(2): 169-173


ISSN/ISBN: 0098-8472
DOI: 10.1016/0098-8472(86)90012-2
Accession: 001311434

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Suspension-cultured tobacco cells were exposed to 44.5, 89, and 178 .mu.M Cd during a 5-day culture period and examined by transmission electron microscopy on days 1, 3, and 5. No structural changes in the mitochondria, cell membranes or in the cytoplasmic density were observed. Nuclei and chromatin appeared unchanged at Cd levels .ltoreq. 89 .mu.M, but a small percentage of the nuclei became irregularly invaginated in cells exposed to 178 .mu.M Cd. The most apparent changes induced by Cd in the tobacco cells were significant increases in cell volume and the formation of numerous small vesicles in the cytoplasm. The significance of these findings, in relationship to the physiological effects of Cd in the tobacco cells is discussed.