+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Calcofluor white detection of fungi in cytopathology



Calcofluor white detection of fungi in cytopathology



American Journal of Clinical Pathology 85(2): 222-225



Calcofluor/Cellufluor (CFW) binds to fungal cell walls and causes them to fluoresce blue-green when illuminated with UV light. Retrospective and prospective studies were made to determine if CFW could be added to the Papanicolaou (PAP) stain procedure without altering diagnostic cytopathologic features while still allowing fungi to be identified. The retrospective study included 136 cytology specimens that were designated positive for fungus by PAP stain; these were stained with a 0.1% aqueous solution of CFW and examined by fluorescent microscopy. The overall agreement between the two methods in the detection of fungi was 90.4%. The incorporation of CFW into the PAP stain was tested at various points in the PAP stain sequence; optimum results were obtained when CFW was introduced after acid eosin. A total of 197 random, sequentially accessioned cytology specimens were stained with the PAP/CFW combination in the prospective study. The results indicate that detection of fungi by a combination of regular light and fluorescent microscopy was far more effective than was examination for the organisms by light microscopy alone.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001311617

Download citation: RISBibTeXText

PMID: 2418677

DOI: 10.1093/ajcp/85.2.222


Related references

Detection of fungi in cytopathology with calcofluor white. American Journal of Clinical Pathology 83(2): 271, 1985

The use of calcofluor white for the detection of fungi which cause subcutaneous and systemic mycoses. Boletin Micologico 14(1-2): 9-12, 1999

Calcofluor white stain for detection of fungi in clinical specimens. Bulletin Postgraduate Institute of Medical Education & Research Chandigarh 25(3): 139-144, 1991

Rapid detection of fungi in tissues using calcofluor white and fluorescence microscopy. Archives of Pathology and Laboratory Medicine 108(8): 616-618, 1984

Clinical laboratory evaluation of the calcofluor white stain for the detection of fungi in clinical specimens. Abstracts of the Annual Meeting of the American Society for Microbiology 85: 378, 1985

Comparison of gram stain potassium hydroxide preparation gomori methenamine silver gms and calcofluor white stain for the detection of fungi in clinical specimens. Abstracts of the Annual Meeting of the American Society for Microbiology 87: 385, 1987

Calcofluor white stain for fungi. American Journal of Clinical Pathology 87(2): 295-296, 1987

Detection of Candida by calcofluor white. Acta Cytologica 54(5): 679-684, 2010

A comparison of the highly selective fluorescence staining of fungi in tissue sections with Uvitex 2B and Calcofluor White M2R. Histochemical Journal 20(4): 215-221, 1988

Calcofluor white stain for detection of pneumocystis carinii. Onkologie 14(Suppl. 2): 3, 1991

Evaluation of calcofluor white stain for detection of Pneumocystis carinii. Diagnostic Microbiology and Infectious Disease 13(4): 307-310, 1990

Evaluation of calcofluor white [CFW] stain for detection of Pneumocystis carinii. Diagnostic Microbiology and Infectious Disease 14(5): 457-458, 1991

Use of calcofluor white for detection, identification, and quantification of phytoplanktonic fungal parasites. Applied and Environmental Microbiology 75(8): 2545-2553, 2009

Detection of Pneumocystis carinii with direct fluorescence antibody and calcofluor white stain. Infection 24(3): 248-250, 1996

The rapid detection of Acanthamoeba in paraffin-embedded sections of corneal tissue with calcofluor white. Archives of Ophthalmology 105(10): 1366-1367, 1987