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Callus induction and detection of metabolites in Tribulus terrestris L

Callus induction and detection of metabolites in Tribulus terrestris L

Journal of Plant Physiology 123(2): 181-186

Callus cultures induced from the leaf and stem portions of Tribulus terrestris L. (Zygophyllaceae) were shown to contain steroidal sapogenins. The influence of some combinations of auxins (2,4-D and NAA) and cytokinins (N6BA and kinetin) on callus induction was investigated. A combination of 2.5 .times. 10-6 M 2,4-D and 2.0 .times. 10-6 M kinetin was found to be best for callus induction and maintenance. Lignin, saponins, fixed oils/fats, flavonoids as well as steroidal sapogenins including ruscogenin, hecogenin, diosgenin in both the free and glycosylated forms in addition to spirosta-3,5-dienes were detected in the callus cultures. While there was evidence to suggest similar biosynthetic potential for the calli and the intact plant, free steroidal sapogenins were absent in the latter.

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Accession: 001311794

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DOI: 10.1016/S0176-1617(86)80139-0

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