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Callus induction from barley microspores. The role of sucrose and auxin in a barley anther culture medium

Callus induction from barley microspores. The role of sucrose and auxin in a barley anther culture medium

Canadian Journal of Botany 63(12): 2209-2212

A barley anther culture basic medium (BAC1) supplemented with 8 mg 2,4-D/litre and 9% sucrose and replenished at 10 day intervals with basic medium containing 1 mg IAA/litre and 3% sucrose was optimal for anther response (21%) and productivity (67 calluses/100 anthers plated) in Elrose.

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Accession: 001311805

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DOI: 10.1139/b85-313

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