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Callus initiation and rhizogenesis in Cucumis sativus L. hypocotyl explants



Callus initiation and rhizogenesis in Cucumis sativus L. hypocotyl explants



Acta Agrobotanicareceived 1986; 36(1/2): 67-81



Hypocotyl explants were cultured on Linsmaier and Skoog or White's basal media supplemented with NAA, IAA or 2,4-D, each at 4 rates, in combination with kinetin. White's medium was not suitable for callus culture or rhizogenesis. Linsmaier and Skoog medium containing NAA at 1.0 or 2.0 mg/litre and kinetin at 0.25 mg/litre gave very good callus growth and a high rooting percentage after 3 weeks of cultivation.

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