Cytogenetic studies on the origin of Allium wakegi Araki. III. Restoration of the fertility of A. wakegi by doubling the chromosome complement
Tashiro, Y.; Miyazaki, S.; Kanazawa, K.; Hashimoto, H.
Bulletin of the Faculty of Agriculture, Saga University 55: 125-129
ISSN/ISBN: 0581-2801 Accession: 001329907
Tetraploids induced by colchicine treatment from a Korean and a Burmese clone of A. wakegi were completely pollen sterile. In crosses of the clones with A. ascalonicum, A. fistulosum or the amphidiploid hybrid between these two, the diploids showed no seed set, while the tetraploids had seed sets of 9-15% and viable seedlings were obtained. The seed set in the Burmese clone was slightly higher than that in the Korean clone. Crosses were more successful when the two species, rather than the amphidiploid hybrid, were used as pollen parents. Both the clones had irregular meiosis, but meiosis was fairly normal in the derived tetraploids. Degeneration of the pollen grain protoplasm was observed in the tetraploids.