+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Diagnosis and immunotherapy of mould allergy. III. Diagnosis of Cladosporium allergy by means of symptom score, bronchial provocation test, skin prick test, RAST, CRIE and histamine release

Diagnosis and immunotherapy of mould allergy. III. Diagnosis of Cladosporium allergy by means of symptom score, bronchial provocation test, skin prick test, RAST, CRIE and histamine release

Allergy 41(1): 57-67

ISSN/ISBN: 0105-4538

PMID: 2421599

DOI: 10.1111/j.1398-9995.1986.tb00276.x

Thirty-three adult asthmatic patients suspected of mould allergy were investigated by in vivo and in vitro tests in order to establish a specific diagnosis of asthma caused by the mould species Cladosporium. The patients were evaluated by daily symptom scores in the peak Cladosporium season, bronchial provocation test (BPT), skin prick test (SPT), RAST, histamine release from basophil granulocytes (HIST), and crossed radioimmunoelectrophoresis (CRIE), and the results were scored as negative (score 0), equivocal (score 1) or positive (score 2). Based on daily symptom scores and the result of BPT the patients were classified as being manifest allergic (asthma) to Cladosporium (positive allergy), inconclusive or negative. Positive allergy was defined as asthma symptoms oscillating with the spore concentration and a BPT score 2 (positive at allergen concentration less than 10,000 BU). Negative allergy was defined as no asthma symptoms and a negative BPT (score 0) and inconclusive in the case of symptoms and BPT sum of score 1-3. According to the classification a final diagnosis (positive or negative) could be established in 85% of the patients. "False positive" tests were found: for BPT in 27%, SPT 18%, RAST 0%, HIST 18%, and CRIE 0%. The corresponding figures for "false negative" were: BPT 0%, SPT 0%, RAST 27%, HIST 18%, and CRIE 23%. The relative risk of being allergic in spite of a negative test result was 0% for BPT and SPT, and 25-30% with RAST, HIST, and CRIE. In the case of positive test the risk was 90-100%. Excluding BPT, SPT was found to be the optimal single test to predict/rule out clinical allergy. A stepwise combination of positive SPT and positive RAST was found exclusively in patients clinically evaluated as positive, and does not call for an additional BPT. Using a potent allergenic extract a negative SPT excluded clinically important allergy. The primary conclusion of the study, however, is that the final diagnosis of Cladosporium asthma could not be based on a positive BPT alone (due to "false positive"), but only on a combination of clinical symptoms during the spore season and a positive BPT.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 001335085

Download citation: RISBibTeXText

Related references

Diagnosis of immunotherapy of mold allergy iii. diagnosis of cladosporium allergy by symptom score bronchial provocation test skin prick test radioallergosorbent test crossed radioimmunoelectrophoresis and histamine release. Allergy 41(1): 57-67, 1986

A new glass microfibre-based histamine analysis for allergy testing in children. Results compared with conventional leukocyte histamine release assay, skin prick test, bronchial provocation test and RAST. Allergy 42(5): 366-373, 1987

Basophil histamine release in the diagnosis of house dust mite and dander allergy of asthmatic children. Comparison between prick test, RAST, basophil histamine release and bronchial provocation. Allergy 45(3): 231-235, 1990

Diagnosis and immunotherapy of mould allergy. II. Reproducibility and relationship between skin sensitivity estimated by end-point titration and histamine equivalent reaction using skin prick test and intradermal test. Allergy 40(5): 354-362, 1985

Allergy diagnosis in patients with bronchial asthma (bronchial provocation test, skin test and RAST). Klinische Wochenschrift 56(24): 1205-1212, 1978

Skin prick test in the diagnosis of dog dander allergy: a comparison of different extracts with clinical history, provocation tests and RAST. Clinical Allergy 10(2): 121-132, 1980

Diagnosis and immunotherapy of mold allergy 2. reproducibility and relationship between skin sensitivity estimated by end point titration and histamine equivalent reaction using skin prick test and intradermal test. Allergy (Copenhagen) 40(5): 354-362, 1985

Sensitivity Comparison of the Skin Prick Test and Serum and Fecal Radio Allergosorbent Test (RAST) in Diagnosis of Food Allergy in Children. Reports of Biochemistry and Molecular Biology 4(2): 98-103, 2016

Pediatric allergy diagnosis pharmacia cap system rast and skin prick test. Clinical and Experimental Allergy 20(Suppl 1): 21, 1990

House dust mite-related allergic diseases: role of skin prick test, atopy patch test, and RAST in the diagnosis of different manifestations of allergy. European Journal of Pediatrics 169(7): 819-824, 2010

Comparison of three prick test methods with the intradermal test and with the rast in the diagnosis of mite allergy. Annals of Allergy 48(4): 235-239, 1982

Specific diagnosis of exogenous bronchial asthma in adults. Comparison between case history, intracutaneous test, RAST, histamine release from basophil leucocytes, and bronchial provocation. Allergy 34(3): 175-185, 1979

The radioallergosorbent test (RAST) in the diagnosis of reaginic allergy : A comparison between provocation tests, skin tests and RAST employing allergo-sorbents which were arbitrarily prepared with commercial allergen extracts. Clinical and Experimental Allergy 4(4): 401-409, 1974

Comparison between fast rast and prick test in the diagnosis of respiratory allergy. Folia Allergologica et Immunologica Clinica 33(4): 285-292, 1986

Value of the radio-allergosorbent test (RAST) in diagnosis of bronchial asthma in children. A comparative clinical trial of skin testing, RAST and bronchial provocation test in 27 children with bronchial asthma. Ugeskrift for Laeger 138(44): 2712-2717, 1976