+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Differential effects of passive immunization with somatostatin antiserum on adenohypophysial hormone secretions in starved rats

Differential effects of passive immunization with somatostatin antiserum on adenohypophysial hormone secretions in starved rats

Journal of Endocrinology 109(2): 169-174

The role of somatostatin (SRIF) on adenohypophyseal hormone secretion in starved rats was reassessed by passive immunization. Because of the absence of pulsatile GH secretion in starved rats, the effects of the injection of SRIF antiserum on GH levels can be clearly demonstrated. To determine whether starvation modifies the sensitivity of the adenohypophysis to SRIF, we measured 125I-labelled iodo-N-Tyr-SRIF binding. There was no difference in the dissociation constant (Kd) nor in the maximal binding capacity (Bmax) in fed (n = 15) and starved (n = 15) animals (Kd = 0.38 .+-. 0.09 (S.E.M.) and 0.45 .+-. 0.09 nmol; Bmax = 204 .+-. 39 and 205 .+-. 30 fmol/mg protein respectively). Administration of SRIF antiserum resulted in a dose-dependent increase in plasma concentrations of GH, TSH and prolactin. The minimal effective dose of SRIF antiserum was 50 .mu.l for GH, 100 .mu.l for TSH and 200 .mu.l for prolactin. Our results show that: (1) starvation does not modify adenohypophyseal SRIF-binding sites, (2) in starved male rats endogenous SRIF exerts a negative control on prolactin secretion in vivo and (3) sensitivity to endogenous SRIF seems to be different for each hypophyseal cell type.

(PDF emailed within 0-6 h: $19.90)

Accession: 001336225

Download citation: RISBibTeXText

PMID: 2872259

DOI: 10.1677/joe.0.1090169

Related references

Increase in basal and thyrotropin-releasing hormone (TRH)-stimulated secretion of thyrotropin (TSH) by passive immunization with antiserum to somatostatin in rats. Endocrinology 98(4): 1069-1072, 1976

Effects of somatostatin and somatostatin antiserum on the passive avoidance behavior and on the afterdischarge in rats after neofrontal decortication. Journal of Endocrinological Investigation 14(6 SUPPL 4): 162, 1991

The effects of somatostatin and somatostatin antiserum on the retention of passive avoidance behavior after neofrontal decortication in rats. Physiology & Behavior 47(5): 1035-1036, 1990

The effects of somatostatin passive immunization of milk yield, plasma hormone and metabolite concentrations in rats. Asian-Australasian Journal of Animal Sciences 10(3): 324-328, 1997

Effects of passive immunization of growth hormone-releasing hormone and somatostatin on growth hormone secretion under conditions of high somatostatin tone. Life Sciences 50(13): 951-958, 1992

Effect of passive immunization against somatostatin using a high-affinity antiserum on growth, IFG-I and thyroid hormone levels in neonatal lambs reared under warm or cold environmental conditions. Canadian Journal of Animal Science 74(1): 23-27, 1994

Passive immunization of fetal rats with antiserum to luteinizing hormone-releasing hormone (LHRH) or transection of the central roots of the nervus terminalis does not affect rat pups' preference for home nest. Physiology and Behavior 41(6): 613-619, 1987

Thyrotropin secretion in starved rats is enhanced by somatostatin antiserum. Hormone and Metabolic Research 16(2): 92-96, 1984

Altered release of growth hormone from dispersed adenohypophysial cells of streptozotocin diabetic rats. I. Effects of growth hormone releasing factor and somatostatin. Canadian Journal of Physiology and Pharmacology 67(10): 1315-1320, 1989

Effects of glucocorticoid treatment and acute passive immunization with growth hormone-releasing hormone and somatostatin antibodies on endogenous and stimulated growth hormone secretion in the male rat. Journal of Endocrinology 131(1): 75-86, 1991

Suppression of serum thyrotropin (TSH) concentrations following thyroidectomy and cold exposure by passive immunization with antiserum to thyrotropin-releasing hormone (TRH) in rats. Metabolism: Clinical and Experimental 27(10): 1485-1490, 1978

Effect of passive immunization to gonadotropin-releasing hormone (GnRH) using GnRH antiserum on the mitotic activity of gonadotrophs in castrated male rats. Endocrinology 122(6): 2803-2808, 1988

Sertoli cell maturation is impaired by neonatal passive immunization with antiserum to luteinizing hormone-releasing hormone. Endocrinology 112(3): 1115-1121, 1983

Effects of adrenal medullectomy and passive immunization with an anti-CRF antiserum on the corticotropin secretion in response to stress in Long-Evans rats. Comptes Rendus de l'Academie des Sciences. Serie Iii, Sciences de la Vie 299(12): 515-520, 1984

Effects of somatostatin antiserum on growth hormone levels in rats with periventricular lesions in the anterior hypothalamus. Brain Research 447(2): 384-388, 1988