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Differentiating pathogenic and non-pathogenic avian influenzavirus in cell culture

Differentiating pathogenic and non-pathogenic avian influenzavirus in cell culture

Berliner und Munchener Tierarztliche Wochenschrift 98(2): 37-39

In cultures of chick-embryo fibroblasts, non-apthogenic strains of avian influenzavirus displayed haemagglutination only in the presence of trypsin; with virulent strains trypsin produced a rise in the haemagglutination titre. In cell cultures overlayed with serum-free agar, non-pathogenic strains formed plaques only in the presence of trypsin at 10-20 micro g/ml, but trypsin had no effect on the formation of plaques by pathogenic strains.

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