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Diffusion barriers for protons at the external surface of thylakoids



Diffusion barriers for protons at the external surface of thylakoids



Advances in photosynthesis research VolII: 261-264



Broken chloroplasts were prepared from 14- to 18-day-old pea seedlings and chloroplasts were stored frozen before being added to the assay medium. Absorption changes were measured using cresol red at 575 nm. Rate of proton uptake from the external bulk phase by PSI was limited by superoxide dismutase and uptake by PSII was predominantly limited by multiple binding and debinding of protons within the grana stacks.

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Accession: 001336466

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