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Digestion and gas production of grass in an artificial rumen



Digestion and gas production of grass in an artificial rumen



Journal of the Faculty of Agriculture Shinshu University 22(1): 37-44



This experiment was conducted with an artificial rumen to examine whether NDF (Neutral Detergent Fiber) digestibility of forage used as substrate can be evaluated by the cumulative gas production during fermentation. The forages used as substrate were white clover, red clover, orchard grass and tall fescue at the vegetative, blooming and fruitage stages and CO2-treated incubation medium (pH 6.9) consisted of goat rumen juice and McDougall's synthetic saliva other than forage substrate. Incubation was performed for 5 hours, 10 hours or 15 hours, and the gas produced was determined by the pipette method. The results obtained are as follows: Cumulative gas production during fermentation was more when white clover or red clover was used as substrate than when orchard grass or tall fescue was used, irrespective of the maturity stages of forages and incubation time. Cumulative gas production, dry matter digestibility and NDF digestibility responded to the maturity of each forage tested in similar manners. Significant high correlations were found between cumulative gas production and NDF digestibility in all forages tested, regardless of the maturity of forages and incubation time, suggesting that NDF digestibility of forage substrate can be evaluated by the cumulative gas production in an artificial rumen.

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Accession: 001336601

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