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Digestion of several temperate forage species by a prepared cellulase solution



Digestion of several temperate forage species by a prepared cellulase solution



Agronomy Journal 78(1): 94-98



Rapid, inexpensive, and accurate procedures are required for predicting in vivo dry matter digestibility (DMD) of forages [Dactylis glomerata, Bromus inermis, Phleum pratense, Medicago sativa] particularly in a forage breeding program when many samples may be analyzed simultaneously. The primary objective of this study was to investigate the relationships of several cellulase digestion procedures to in vivo DMD, in vitro DMD using rumen liquor, neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and hemicellulose for several temperature forage species. A cellulase solution was produced by culturing the conidia from Trichoderma reesi Simmons in a mineral medium containing 10 g L-1 cellulose. Apparent activity of the cellulase solution was determined by digesting cool season forages of known DMD and solka floc substrate. Forty-two dried cool season forages which previously had been evaluated for per cent NDF, ADF, ADL, in vitro DMD using rumen liquor, and in vivo DMD using sheep were subjected to different cellulase digestion procedures. Correlations between the various cellulase DMD techniques and in vivo DMD were highly significant (p < 0.01) and ranged from 0.89 to 0.97. Correlations of the cellulase in vitro DMD techniques and NDF, ADF, ADL, and hemicellulose were negative and similar in magnitude to the same parameters when correlated to in vitro DMD using rumen liquor. The data suggested that the in vitro DMD obtained by using a prepared cellulase solution was an excellent predictor of in vivo DMD and the digestion was similar to that when digesting forages with rumen liquor.

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Accession: 001336635

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