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Diploidization of plant tissue cultures by means of 5-uracilyl thioureidoglucose (thiacil)



Diploidization of plant tissue cultures by means of 5-uracilyl thioureidoglucose (thiacil)



Doklady Akademii Nauk SSSR 279(5): 1241-1244



Inclusion of this growth regulator in the nutrient medium had a diploidizing effect on tetraploid cultures of Haplopappus gracilis and Rauwolfia serpentina, and also reduced the degree of polyploidization observed in cultures of Nicotiana tabacum Samsun, so that after 12 passages the control tobacco (with kinetin in Linsmaier & Skoog medium) had >95% polyploid cells while the culture with 5 mg thiacil/litre had predominantly diploid cells.

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