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Direct evidence for de-novo synthesis of LH receptors in cultured pig granulosa cells in response to FSH



Direct evidence for de-novo synthesis of LH receptors in cultured pig granulosa cells in response to FSH



Journal of Reproduction and Fertility 73(2): 343-351



Granulosa cells were cultured in Medium 199 plus 10% pig serum, with or without 0.1 micro g FSH/ml; 1 mU insulin/ml, 0.01 micro g cortisol/ml and thyroxine (10-7M) added to each culture. FSH plus insulin, cortisol, and thyroxine stimulated incorporation of dense isotope-containing (superscript 2H, 13C, 15N) amino acids into soluble 125I-labelled HCG binding sites. Evidence of de novo synthesis of binding sites appeared within 3 h after the beginning of the pulse-labelling period.

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Accession: 001336896

Download citation: RISBibTeXText

PMID: 2985778

DOI: 10.1530/jrf.0.0730343



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