+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Direct gene transfer to plants



Direct gene transfer to plants



Embo Journal 3(12): 2717-2722



Evidence for direct, gene-mediated stable genetic transformation of plant cells of Nicotiana tabacum is presented. A selectable hybrid gene comprising the protein coding region of the Tn5 aminoglycoside phosphotransferase type II gene under control of cauliflower mosaic virus gene VI expression signals was introduced into plant protoplasts as part of an Escherichia coli plasmid. The gene was stably integrated into plant genomic DNA and constitutively expressed in selected, drug resistant, protoplast-derived cell clones. The mode of integration of the foreign gene into the plant genome resembled that observed for DNA transfection of mammalian cells. Plants regenerated from transformed cell lines were phenotypically normal and fertile, and they maintained and expressed the foreign gene throughout the development of vegetative and generative organs. Microspores, grown in anther culture, developed into resistant and sensitive haploid plantlets. Genetic crossing analysis of one of the transformed plants revealed the presence of one dominant trait for kanamycin resistance segregating in a Mendelian fashion in the F(1) generation.

(PDF emailed within 0-6 h: $19.90)

Accession: 001336906

Download citation: RISBibTeXText

PMID: 16453573

DOI: 10.1016/0168-9525(85)90015-0



Related references

Direct gene transfer to plants and inheritance of foreign genes in transgenic plants. Acta Biologica Hungarica 37(Supplementum): 42, 1986

Gene rescue in plants by direct gene transfer of total genomic DNA into protoplasts. Nucleic Acids Research 20(15): 3977-3982, 1992

Plant gene vectors and genetic transformation dna mediated direct gene transfer to plants. Schell, J And I K Vasil (Ed ) Cell Culture And Somatic Cell Genetics Of Plants, Vol 6 Molecular Biology Of Plant Nuclear Genes Xxvi+494p Academic Press, Inc : Troy, Missouri, Usa; London, England, Uk Illus 51-68, 1990

Direct gene transfer to plants. Plant DNA infectious agents edited by Th Hohn and J Schell: 247, 1987

Chemical methods for direct gene transfer to protoplasts. II: Recovery of stable transformants after direct gene transfer using polyethylene glycol (PEG). Maize Genetics Cooperation News Letter ( 63): 23-24, 1989

Localization by in situ hybridization of a low copy chimaeric resistance gene introduced into plants by direct gene transfer. Molecular and General Genetics 207: 204-209, 1987

Methods for direct gene transfer to plants. Plant Physiology and Biochemistry, France 25(3): 361-364, 1987

Direct gene transfer to plants. 1984. Biotechnology 24: 387-392, 1992

Direct gene transfer to plants: facts and future. Plant biology(3): 289-302, 1986

Direct visualization of horizontal gene transfer in cotton plants. Journal of Heredity 105(6): 834-836, 2015

Lipofectin: direct gene transfer to higher plants using cationic liposomes. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 83(1): 1-5, 1991

Direct gene transfer to plants: present developments and some future prospectives. Plant Physiology and Biochemistry, France 25(4): 493-503, 1987

Molecular analysis of transgenic rapeseed plants obtained by direct transfer of two separate plasmids containing, respectively, the cauliflower mosaic virus coat protein gene and a selectable marker gene. Plant Science (Limerick) 91(2): 181-193, 1993

Structure and expression of tomato mitochondrial genes coding for cysteine transfer rna gca asparagine transfer rna guu and tyrosine transfer rna gua a native cysteine transfer rna gene is present in dicot plants but absent in monocot plants. Current Genetics 18(3): 239-244, 1990