EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Direct measurements of steady-state kinetics of cyanobacterial n(2) uptake by membrane-leak mass spectrometry and comparisons between nitrogen fixation and acetylene reduction



Direct measurements of steady-state kinetics of cyanobacterial n(2) uptake by membrane-leak mass spectrometry and comparisons between nitrogen fixation and acetylene reduction



Applied and Environmental Microbiology 45(4): 1331-1337



A mass spectrometer with a membrane-covered inlet was used to measure nitrogen fixation by following changes in the concentration of dissolved N2 in a stirred suspension of the cyanobacterium Anabaena variabilis in an open system. The results showed a good fit to Michaelis-Menten kinetics with a Km for N2 of 65 mu M at 35 degrees C, corresponding to 0.121 atmosphere of N2. Corresponding values for the Km for acetylene reduction were 385 mu M (0.011 atmosphere at 35 degrees C).

(PDF emailed within 1 workday: $29.90)

Accession: 001336926

Download citation: RISBibTeXText

PMID: 16346272



Related references

Direct measurements of steady state kinetics of cyanobacterial nitrogen uptake by membrane leak mass spectrometry and comparisons between nitrogen fixation and acetylene reduction. Applied & Environmental Microbiology 45(4): 1331-1337, 1983

Direct measurements of steady-state kinetics of cyanobacterial N2 nitrogen uptake by membrane-leak mass spectrometry and comparisons between nitrogen fixation and acetylene reduction Algae. 1983

Mass spectrometric measurements of steady state kinetics of cyanobacterial nitrogen fixation by monitoring dissolved nitrogen in an open system. FEMS Microbiology Letters 12(1): 37-40, 1981

Mass spectrometric measurements of steady-state kinetics of cyanobacterial nitrogen fixation by monitoring dissolved N2 in an open system. Fems Microbiology Letters 12(1): 37-40, 1981

Biological nitrogen fixation (acetylene reduction) associated with blue-green algal (cyanobacterial) communities in the Beachwood Mangrove Nature Reserve II. Seasonal variation in acetylene reduction activity. South African Journal of Botany 59(1): 1-8, 1993

Biological nitrogen fixation (acetylene reduction) associated with blue-green algal (cyanobacterial) communities in the Beachwood Mangrove Nature Reserve. I. The effect of environmental factors on acetylene reduction activity. South African Journal of Botany 55(4): 438-446, 1989

Biological nitrogen fixation acetylene reduction associated with blue green algal cyanobacterial communities in the beachwood mangrove nature reserve south africa i. the effect of environmental factors on acetylene reduction activity. South African Journal of Botany 55(4): 438-446, 1989

Cyanobacterial dinitrogen uptake measurements using membrane-inlet mass spectrometry. Special ications of the Society for General Microbiology4(14): 279-285, 1985

Kinetics of nitrogen fixation and acetylene reduction and effects of oxygen and of acetylene on these processes in a soil system. Bulletins from the Ecological Research Committee-NFR (Statens Naturvetenskapliga Forskningsrad) 17: 255-262, 1972

Kinetics of nitrogen fixation and acetylene reduction, and effects of oxygen and of acetylene on these processes, in a soil system. "Modern Methods in the Study of Microbial Ecology" Bulletins from the Ecological Research Committee, Sweden 17: 255-262, 1973

Kinetics of base catalyzed deuterium hydrogen exchange in aqueous solutions of acetylene measurements using membrane inlet mass spectrometry. Acta Chemica Scandinavica 45(3): 308-315, 1991

Nitrogen fixation acetylene reduction in nonheterocystous cyanobacterial mats from the dampier archipelago western australia. Australian Journal of Marine & Freshwater Research 40(2): 147-154, 1989

Relationships between acetylene reduction activity hydrogen evolution and nitrogen fixation in nodules of acacia spp experimental background to assaying fixation by acetylene reduction under field conditions. Journal of Experimental Botany 38(186): 1-12, 1987

Field measurements of symbiotic nitrogen fixation in an established pasture using acetylene reduction and a nitrogen 15 method. Soil Biology and Biochemistry 10(1): 13-20, 1978

Comparisons of nitrogen fixation estimates in soybeans by nodule weight, leghemoglobin content, and acetylene reduction. Canadian Journal of Microbiology 19(9): 1165-1168, 1973