+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Direct solid-phase enzyme immunoassay of progesterone in saliva

Direct solid-phase enzyme immunoassay of progesterone in saliva

Clinical Chemistry 30(9): 1507-1511

We have developed a rapid enzyme immunoassay for progesterone in saliva. This solid-phase assay is carried out on microtitre plates with no extraction or centrifugation steps. The detection limit of the assay is 200 fg per well (3.2 pmol/L). Intra- and interassay coefficients of variation for low, medium, and high concentrations of progesterone were 7.5, 16.0; 9.1, 8.3; and 8.7, 6.7%, respectively. Correlation between total plasma progesterone (assayed by enzyme immunoassay with extraction) and salivary progesterone concentrations was good (r = 0.848, p less than 0.001, n = 56). We found the assay useful for monitoring ovarian function. The analytical procedure is convenient, and one person can assay more than 200 saliva samples per working day. The turnaround time for 36 samples is 2 h, including 1.5 h of incubation time, when previously coated plates are used. We conclude that such assays are very suitable for measuring progesterone in serial saliva samples and could become the preferred method for monitoring ovarian function.

(PDF emailed within 1 workday: $29.90)

Accession: 001336944

Download citation: RISBibTeXText

PMID: 6380813

Related references

Ovulation detection by measurement of progesterone in saliva using a direct solid phase enzyme immunoassay. Archives of Gynecology 237(SUPPL): 93, 1985

Direct enzyme immunoassay of progesterone in human serum by solid phase. Annals of Clinical Biochemistry 24(SUPPL 2): 166-167, 1987

A solid phase enzyme immunoassay for progesterone. National Science Council Monthly 11(12 PART 3): 1553-1558, 1984

Enzyme immunoassay for digoxin (solid phase ELISA) in serum and saliva. Zeitschrift für Medizinische Laboratoriumsdiagnostik 27(8): 435-442, 1986

A simple solid phase enzyme immunoassay for aldosterone in plasma and saliva. Experimental and Clinical Endocrinology 82(2): 188-193, 1983

Direct solid-phase chemiluminescence immunoassay for salivary progesterone. Clinical Chemistry 32(5): 763-767, 1986

Solid-phase enzyme immunoassay for milk progesterone. Acta Biochimica et Biophysica Sinica 20(4): 428-433, 1988

A sensitive solid phase enzyme immunoassay for testosterone in human plasma and saliva. Journal of Endocrinology 81(2): 165p-165p, 1979

Direct no extraction assay for serum progesterone by solid phase iodine 125 radio immunoassay. Clinical Chemistry: 1613-1614, 1982

A solid-phase enzyme immunoassay for the determination of progesterone in bovine, porcine and ovine plasma. Canadian Journal of Animal Science 75(4): 525-529, 1995

Comparison of solid phase enzyme immunoassay solid phase radio immunoassay and density gradient centrifugation for demonstration of anti hepatitis associated virus antibody of the immuno globulin m class. Gastroenterology 76(5 PART 2): 1298, 1979

On the objective evaluation of the results of direct solid phase enzyme immunoassay for antigen identification. Voprosy Virusologii 31(5): 573-577, 1986

Reliability and practical values of a polystyrene bead-solid phase enzyme immunoassay kit for determining the progesterone concentration in defatted cow's milk. Japanese Journal of Animal Reproduction 33(2): 64-72, 1987

Results of using the direct solid phase enzyme immunoassay for the evaluation of the specific activity of rabies vaccines. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii (5): 30-33, 1991

Enzyme immunoassay of progesterone in saliva. Irish Journal of Medical Science 153(6): 195-197, 1984