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Effect of antiserum treatment on the production of virus-free Cymbidium by means of meristem culture


, : Effect of antiserum treatment on the production of virus-free Cymbidium by means of meristem culture. Nogaku Kenkyu 60(3): 123-133

When pieces (1 mmsuperscript 3) of the bulb of Odontoglossum ringspot virus-infected Cymbidium were immersed for 2 h or 1 day in ORSV antiserum (titre 1:2048) the treatment markedly decreased the pathogenicity of extracted sap. Very few virus-free plants could be obtained by meristem culture, only a small piece without leaf primordia being useful for this purpose, but following immersion for 1 h in ORSV antiserum 52.9% of the plants from meristem culture were virus-free.

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