+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evidence for the occurrence of respiratory electron transport in adult Brugia pahangi and Dipetalonema viteae



Evidence for the occurrence of respiratory electron transport in adult Brugia pahangi and Dipetalonema viteae



Molecular and Biochemical Parasitology 143: 337-354



Mixed-sex adult stages of Brugia pahangi and Dipetalonema viteae, in the absence of exogenous substrate, consumed oxygen at rates of 4.18 +/- 0.38 and 2.12 +/- 0.20 ngatoms O2 min-1 mg-1 dry wt. respectively. When calculated on a unit dry weight basis the endogenous O2 consumption rates (E-QO2) of mature adult male macrofilariae of B. pahangi and D. viteae were significantly greater than those of mature females, although the E-QO2 calculated per individual worm was essentially similar irrespective of sex. When assayed as separate unisexual groups, the oxygen uptake of male and female macrofilariae of both species was inhibited by classical inhibitors of respiratory electron transport (RET), and showed classical substrate bypass phenomena in response to succinate and ascorbate, N,N,N',N'-tetramethyl-p-phenylenediamine with respect to the RET inhibitors rotenone (inhibitor of complex I) and antimycin A (inhibitor of complex III). Since male worms elicited similar responses to the classical RET inhibitors as did mixed-sex and/or adult female populations, the possibility that developmental stages contained within the female filariids were contributing in any significant manner to the overall responses observed with the RET inhibitors can be discounted. Such responses as observed with live-intact macrofilariae are normally elicited only by mitochondrial preparations and suggest that the cuticles of both species are permeable to rotenone, succinate, antimycin A, N,N,N',N'-tetramethyl-p-phenylenediamine, azide and cyanide. The uncoupler 2,4-dinitrophenol stimulated the endogenous rate of oxygen consumption (E-QO2) of intact B. pahangi at 33-160 microM, indicating the probable occurrence of RET-coupled oxidative phosphorylation. Higher concentrations of 2,4-dinitrophenol proved inhibitory. Respiratory studies on subcellular fractions substantiated the responses elicited by the intact parasites, suggesting the presence of antimycin A-sensitive and -insensitive RET pathways capable of utilising alpha-glycerophosphate, succinate, and malate as substrates. Both B. pahangi and D. viteae macrofilariae therefore probably possess branched RET-pathways bifurcating on the substrate side of RET-complex III. The rates of substrate oxidation in terms of QO2 mg-1 mitochondrial protein compare well with those observed with other nematode parasites.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001363048

Download citation: RISBibTeXText

PMID: 4039407

DOI: 10.1016/0166-6851(85)90061-1


Related references

Evidence for the occurrence of respiratory electron transport in dipetalonema viteae and brugia pahangi macrofilariae. Parasitology 89(2): LXXVIII, 1984

Oral transmission of Brugia pahangi and Dipetalonema viteae to adult and neonatal jirds. International Journal for Parasitology 6(1): 75-78, 1976

A comparison of the response of Dipetalonema viteae and Brugia pahangi adult worms to antifilarial agents in vitro. Tropical Medicine and Parasitology 37(4): 375-380, 1986

Studies on the humoral immune response of Mastomys natalensis to experimental filarial infections (Litomosoides carinii, Dipetalonema viteae, Brugia malayi and Brugia pahangi). Untersuchungen zur humoralen Immunreaktion von Mastomys natalensis im Verlauf experimenteller Filarieninfektionen Litomosoides carinii, Dipetalonema viteae, Brugia malayi und Brugia pahangi: [xii] + 272, 1985

Reaginic and homocytotropic igg antibody response of mastomys natalensis in experimental infections of filarial parasites litomosoides carinii dipetalonema viteae brugia malayi brugia pahangi. Parasitology Research 73(3): 271-280, 1987

Studies on the humoral immune response of Mastomys natalensis in the courses of experimental filarial infection (Litomosoides carinii, Dipetalonema viteae, Brugia malayi and Brugia pahangi). 1985

Physicochemical characteristics of non-electrolytes and their uptake by Brugia pahangi and Dipetalonema viteae. Molecular and Biochemical Parasitology, 272-3: 101-108, 1988

Studies on Brugia pahangi and Dipetalonema viteae in the jird, Meriones unguiculatus. Dissertation Abstracts International 34B(8): 3640, 1974

An investigation of the glucose metabolism of brugia pahangi and dipetalonema viteae by nmr spectroscopy. Molecular & Biochemical Parasitology 18(2): 171-182, 1986

Dipetalonema viteae and Brugia pahangi transplant infections in gerbils for use in antifilarial screening. Journal of Helminthology 62(1): 1-9, 1988

Comparative utilization of pyruvate by Brugia pahangi, Dipetalonema viteae, and Litomosoides carinii. Journal of Parasitology 65(1): 1-7, 1979

Time courses of antibody levels in mastomys natalensis after infections with litomosoides carinii dipetalonema viteae brugia malayi or brugia pahangi determined by elisa enzyme linked immuno sorbent assay. Zeitschrift fuer Parasitenkunde 69(5): 681-692, 1983