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Excision of the Ds controlling element from the Adh1 gene of maize



Excision of the Ds controlling element from the Adh1 gene of maize



Maydica 31(1): 47-57



Revertants of the Ds-induced mutant of Adh1 (alcohol dehydrogenase), Adh1Fm335, which has enzyme activity 15% of that of the wild type, were isolated at a frequency of about 3% from plants containing Ac. Of 8 revertant alleles characterized, 7 showed imprecise excision of the Ds element, while one showed precise excision. In cases of imprecise excision, bases from the flanking duplication remained, with base changes and/or deletions induced at the point of excision.

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