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Exposure to different cryoprotectants, freezing, incubation and vital staining of rabbit embryos and blastomeres

Exposure to different cryoprotectants, freezing, incubation and vital staining of rabbit embryos and blastomeres

Sel' skokhozyaistvennaya Biologiya (3): 101-104

Rabbit embryos at the 2- to 8-cell stages in 1.6 or 1.8 M DMSO were frozen rapidly or slowly in liquid nitrogen, and blastomeres derived from 2- to 16-cell embryos were frozen in 1.5-1.8 M DMSO, or in 0.5 M or 1.0 M dimethylacetamide. Embryos and blastomeres were thawed at 5 degrees -14 degrees C or 25 degrees per min, incubated for 24-30 h in modified medium 199, and stained with neutral red. All embryos and isolated blastomeres were dead after thawing.

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