+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Expression of foreign genes in regenerated plants and in their progeny



Expression of foreign genes in regenerated plants and in their progeny



Embo Journal 3(8): 1681-1689



Chimeric genes comprised of the nopaline synthase promoter and bacterial coding sequences specifying resistance to kanamycin, chloramphenicol or methotrexate, were inserted into the non-oncogenic Ti plasmid vector pGV3850 by recombination (through homologous pBR322 sequences present in the chimeric gene constructs and pGV3850). These co-integrates in Agrobacterium were used to infect single plant protoplasts of Nicotiana by co-cultivation. The resistance traits allowed the selection of transformed calli in tissue culture in the presence of the appropriate antibiotic. Furthermore, as a non-oncogenic Ti plasmid was used for the protoplast transformation, phenotypically normal and fertile plants could be regenerated from the resistant calli. We have shown that these fully differentiated plant tissues exhibit functional expression of resistance traits (Km and Cm). All plants carrying the chimeric genes developed normally, flowered, and set seeds. The inheritance of several of these resistance traits was analyzed and shown to be Mendelian. These results are model experiments to demonstrate that genes of interest can be systematically transferred to the genome of plants using non-oncogenic Ti plasmid derivatives; and that transformed plants are capable of normal growth and differentiation, thus providing a natural environment for the study of gene expression and development of plant cells.

(PDF emailed within 1 workday: $29.90)

Accession: 001364660

Download citation: RISBibTeXText

PMID: 16453538


Related references

Selection of amitrole tolerant tobacco calli and the expression of this tolerance in regenerated plants and progeny. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 67(5): 427-432, 1984

Selection of amitrole tolerant tobacco nicotiana tabacum cultivar wisconsin 38 calli and the expression of this tolerance in regenerated plants and progeny. Theoretical & Applied Genetics 67(5): 427-432, 1984

The expression of introduced genes in regenerated plants. Genetic engineering: Principles and methods(9): 45-59, 1987

Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Tsitologiia i Genetika 39(6): 9-14, 2006

High level expression of introduced chimeric genes in regenerated transformed plants. EMBO (European Molecular Biology Organization) Journal 4(10): 2411-2418, 1985

Problems that can limit the expression of foreign genes in plants: lessons to be learned from B.t. toxin genes. Genetic Engineering 18: 83-99, 1996

First results on the progeny of regenerated plants. Maize Genetics Cooperation Newsletter (61): 75-76, 1987

Variability among plants and their progeny regenerated from protoplasts of Su. Theoretical and applied genetics6(1): 67-75, 1983

A new allele of vp5 isolated in the progeny of regenerated plants. Maize Genetics Cooperation Newsletter (62): 96, 1988

A new allele of sr2 isolated in the progeny of regenerated plants. Maize Genetics Cooperation Newsletter ( 67): 79, 1993

Cytogenetic variation in rye regenerated plants and their progeny. Genome. 35(3): 428-430, 1992

Analysis of the progeny of regenerated plants of sorghum. Fiziologicheskie i geneticheskie osnovy selektsii: 191-201, 1984

Genetic variants in progeny of regenerated maize plants. Genome 29(6): 834-838, 1987

Discovery of Ac activity among progeny of regenerated maize plants. Maize Genetics Cooperation Newsletter (59): 91, 1985