+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Extracellular proteins associated with induction of differentiation in bean rust uredospore germlings

Extracellular proteins associated with induction of differentiation in bean rust uredospore germlings

Phytopathology 75(9): 1073-1076

The bean rust fungus (Uromyces appendiculatus) has a contact-sensitive response to the stoma, the fungal penetration site on a leaf. Stomatal recognition is followed by mitotic nuclear division and differentiation of the first infection structure, the appressorium. Our evidence suggests that proteins in the extracellular matrix of the germ tube may be involved in the thigmotropic response. Nuclear division of germlings incubated in vitro on a normally inductive scratched surface was significantly reduced by 500 .mu.g of either proonase E or trypsin per milliliter but not by heat-denatured enzymes, trypsin mixed with trypsin inhibitor, or 500 .mu.g of either .alpha.- or .beta.-glucosidase, .alpha.-mannosidase, or lipase per milliliter. Incubating germlings with pronase E at any time prior to nuclear division inhibited germling response to the scratched surface. Pronase E at 200 .mu.g/ml did not decrease rates of germination or germ tube elongation, but adhesion of the germlings to the substrate was significantly reduced. The data suggest that extracellular proteins may bind the germ tube to an inductive surface, and that binding may be necessary for induction of infection structures.

(PDF emailed within 1 workday: $29.90)

Accession: 001364874

Download citation: RISBibTeXText

Related references

A possible role for microtubules and microfilaments in the induction of nuclear division in bean rust uredospore germlings. Experimental Mycology 6(3): 293-302, 1982

Changes in DNA content of nuclei in rust uredospore germlings during the start of differentiation. Experimental Mycology 8(3): 245-255, 1984

DNA synthesis and nuclear division during formation of infection structures by bean rust uredospore germlings. Archives of Microbiology 104(2): 123-127, 1975

Heat shock induced development of infection structures by bean rust uredospore germlings. Experimental Mycology 13(2): 149-157, 1989

Cyclic amp cyclic gmp and bean rust uredospore germlings. Experimental Mycology 13(1): 100-104, 1989

Metabolic alterations in bean rust germlings during differentiation induced by the potassium ion. Experimental Mycology 8(3): 183-192, 1984

Analysis of a developmental gene expressed during differentiation of germlings of the bean rust fungus. Molecular genetics of plant microbe interactions 1988: proceedings of the 4th International Symposium on Molecular Genetics of Plant Microbe Interactions Acapulco Mexico May 15-20-1988 edited by Rafael Palacios: 272, 1988

Detailed characterization of a genomic clone specifically expressed during differentiation of bean rust germlings. Journal of Cellular Biochemistry Supplement (12 PART C): 276, 1988

A sensing mechanism in rust Uredospore germlings responsive to host morphology starts the cell cycle. Infection processes of fungi: a Bellagio Conference: March 21-25-1983 edited by Donald W Roberts and James R Aist: 136, 1984

Terminology to describe the differentiation response by germlings of fungal spores Example with bean rust, Uromyces phaseoli. Phytopathology 73(3): 380, 1983

Role of cyclic amp in differentiation of uromyces phaseoli uredospore germlings. Phytopathology 74(7): 832, 1984

Synthesis of differentiation-specific proteins in germlings of the wheat stem rust fungus after heat shock. Experimental Mycology 9(3): 279-283, 1985

Cyclic nucleotides and bean rust uredospore development. Phytopathology 76(10): 1128, 1986

Effect of potassium on sugar uptake and assimilation by bean rust germlings. Mycologia 77(2): 248-252, 1985

Potassium ion induces bean rust germlings to develop infection structures. Phytopathology 72(7): 957, 1982