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Extrachromosomal DNA transformation of C. elegans

Extrachromosomal DNA transformation of C. elegans

Genetic manipulation of the early mammalian embryo: 251-263

The genome of C. elegans was transformed by microinjecting DNA into the cytoplasm of the syncytial gonad. Cytoplasmic degradation did not appear to be a limiting factor for transformation; transformants were obtained in wild-type and nuclease-deficient gonads at comparable frequencies. Foreign DNA was detected amongst the progeny in approximately 10% of the injections. The exogenous DNA was present as a high molecular weight array.

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Accession: 001364879

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