EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Extrachromosomal DNA transformation of C. elegans



Extrachromosomal DNA transformation of C. elegans



Genetic manipulation of the early mammalian embryo: 251-263



The genome of C. elegans was transformed by microinjecting DNA into the cytoplasm of the syncytial gonad. Cytoplasmic degradation did not appear to be a limiting factor for transformation; transformants were obtained in wild-type and nuclease-deficient gonads at comparable frequencies. Foreign DNA was detected amongst the progeny in approximately 10% of the injections. The exogenous DNA was present as a high molecular weight array.

(PDF 0-2 workdays service: $29.90)

Accession: 001364879

Download citation: RISBibTeXText



Related references

Extrachromosomal dna transformation of caenorhabditis elegans. Costantini, F And R Jaenisch (Ed ) Banbury Report, 20 Genetic Manipulation Of The Early Mammalian Embryo; Conference, Oct 1984 Xi+289p Cold Spring Harbor Laboratory: Cold Spring Harbor, N Y , Usa Illus 251-264, 1985, 1986

Extrachromosomal DNA transformation of Caenorhabditis elegans. Molecular and Cellular Biology 5(12): 3484-3496, 1985

Genetic transformation of somatic cells. III. An analysis of the status of the plasmid nucleotide sequences in the extrachromosomal DNA of transformant clone cells and the rescue of extrachromosomal molecules of the plasmid DNA. Tsitologiia 27(5): 565-571, 1985

Extrachromosomal copies of transposon Tc1 in the nematode Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America 81(13): 4018-4022, 1984

Gene editing activity on extrachromosomal arrays in C. elegans transgenics. Gene 475(2): 87-93, 2011

Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences. Embo Journal 10(12): 3959-3970, 1991

Efficient gene transfer in caenorhabditis elegans extrachromosomal maintenance and integration of transforming sequences. EMBO (European Molecular Biology Organization) Journal 10(12): 3959-3970, 1991

A rapid protocol for integrating extrachromosomal arrays with high transmission rate into the C. elegans genome. Journal of Visualized Experiments 82): E50773-E50773, 2014

Analysis of the VPE sequences in the Caenorhabditis elegans vit-2 promoter with extrachromosomal tandem array-containing transgenic strains. Molecular and Cellular Biology 14(1): 484-491, 1994

Mosaic analysis using a ncl-1 (+) extrachromosomal array reveals that lin-31 acts in the Pn.p cells during Caenorhabditis elegans vulval development. Genetics 143(3): 1181-1191, 1996

Association of transformation of xenosomes from nonkiller to killer with extrachromosomal DNA. Journal of Bacteriology 168(1): 96-102, 1986

Stable transformation of Leptomonas seymouri by circular extrachromosomal elements. Proceedings of the National Academy of Sciences of the United States of America 88(15): 6711-6715, 1991

Cervical keratinocytes containing stably replicating extrachromosomal HPV-16 are refractory to transformation by oncogenic H-Ras. Virology 356(1-2): 68-78, 2006

Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation. Embo Journal 9(10): 3077-3084, 1990