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Extraction and determination of adenosine 5'-triphosphate in bovine milk by the firefly luciferase assay



Extraction and determination of adenosine 5'-triphosphate in bovine milk by the firefly luciferase assay



Biotechnology and Applied Biochemistry 8(5): 361-369



Release of ATP from somatic cells in milk with Triton X-100 was optimized for assay with firefly luciferase. A small vol. of milk (25 micro l) was added to 0.8 ml of 0.2% Triton X-100 in 100 mM Tris, 4 mM EDTA, pH 7.8. After approx. 1 min, 0.2 ml luciferase reagent was added and emission of light was measured in a luminometer. Results were calibrated with an ATP standard. This simple method gave high yields of ATP from somatic cells in milk without interference from bacterial ATP.

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Accession: 001364908

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PMID: 3768145



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