Fluorescence intensity of strains of nodule bacteria (Rhizobium meliloti and Rhizobium phaseoli) differing in activity, grown in the presence of the lipophilic vital stain phosphine 3R

Bonartseva, G.A.; Myshkina, V.L.

Microbiology 54(4): 535-541


ISSN/ISBN: 0026-2617
Accession: 001369823

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A fluorometric method of determining the content of poly- beta -hydroxybutyrate in cells of nodule bacteria according to the fluorescence intensity of cells grown in the presence of a lipophilic vital stain phosphine 3R, is described. The fluorescence intensity of the microbial suspension was measured on a spectro-fluorometer at a maximum wavelength of excitation 380 nm and a maximum wavelength of fluorescence 470 nm. The method can be used for a differentiation of strains of nodule bacteria with high and low activity according to the accumulation of poly- beta -hydroxybutyric acid (PHBA) when they are grown in pure cultures. Relatively inactive strains possess a high peak of fluorescence at 470 nm; in strains with medium activity the fluorescence peak is lower; active strains possess virtually no fluorescence peak at set radiation wavelength. The possibilities of using the method of fluorometric analysis of cells of microorganisms vitally stained with phosphine 3R to study formed granules of PHBA are discussed.