Glyoxysomal Malate Synthase of Cucumber: Molecular Cloning of a cDNA and Regulation of Enzyme Synthesis during Germination
Smith, S.M.; Leaver, C.J.
Plant Physiology 81(3): 762-767
ISSN/ISBN: 0032-0889 PMID: 16664899 DOI: 10.1104/pp.81.3.762
Using a cDNA clone for glyoxysomal malate synthase from cucumber cotyledons as a probe, it was shown that malate synthase gene transcripts in cucumber cotyledons increased in amount to a peak on the 3rd or 4th day of germination and then declined. The rate of decline was greater in the light than in the dark. Direct assays of enzyme activity and immunological tests indicated that the regulation of malate synthase was brought about by changes in the number of gene transcripts for the enzyme rather than through a control of translation.