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Growth and regeneration of alfalfa callus lines after freezing in liquid nitrogen



Growth and regeneration of alfalfa callus lines after freezing in liquid nitrogen



Plant Science, Irish Re ic 42(2): 133-140



Cultured callus lines of Medicago sativa established from the cotyledons of aseptically germinated seeds were treated with a cryoprotective mixture containing 10% (w/v) polyethylene glycol, 8% (w/v) glucose and 10% (w/v) DMSO, frozen at 1 degrees /min to -30 degrees C, and transferred to liquid nitrogen (LN). Samples in glass tubes were thawed rapidly, rinsed with a simplified Murashige & Skoog medium and placed on a modified Blaydes agar medium for growth comparisons.

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Accession: 001378042

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DOI: 10.1016/0168-9452(85)90153-0


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