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Guard cell regulation of stomatal response: chloroplast function and abscisic acid metabolism in guard cells of Commelina communis and Vicia faba



Guard cell regulation of stomatal response: chloroplast function and abscisic acid metabolism in guard cells of Commelina communis and Vicia faba



Dissertation Abstracts International, B Sciences and Engineering 45(1): 17



Methods of isolating guard cells by sonication of epidermal peels in V. faba and treatment of epidermal peels at pH 4 in C. communis are described. The V. faba guard cells, which were 98% free of non-guard cell chlorophyll, showed DCMU-sensitive photosystem II activity and had competent electron transport and photophosphorylation. C. communis guard cells metabolized ABA to phaseic acid, dihydrophaseic acid and alkali-labile conjugates. V. faba guard cells formed only the conjugates from ABA.

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Accession: 001378825

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