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In vitro cultivation of African stocks of Trypanosoma (Trypanozoon) brucei evansi

In vitro cultivation of African stocks of Trypanosoma (Trypanozoon) brucei evansi

Annales de la Societe Belge de Medecine Tropicale 66(2): 145-151

Three stocks of T. evansi were successfully cultivated over a monolayer of pig fibroblasts at 37 degrees C using media consisting of RPMI 1640, 20% (v/v) heat-inactivated horse serum and 10% (v/v) Leibovitz medium. Cultured parasites were morphologically indistinguishable from trypanosomes in the bloodstream of the host. One stock was cultured for 55 days and was successfully subcultured. The 2 other stocks were maintained for 35 days only.

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Accession: 001386955

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PMID: 3753050

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