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Isolation of dark tobacco breeding lines differing in alkaloid concentration via anther derived haploids

Isolation of dark tobacco breeding lines differing in alkaloid concentration via anther derived haploids

Zeitschrift fur Pflanzenzuchtung 96(3): 224-231

Haploid plants were generated via anther culture from 5 advanced breeding lines segregating at loci controlling alkaloid production. Haploid plants were screened for alkaloid concentration and diploids of selected haploids were obtained using midvein explants. Progeny from 172 doubled haploids were grown in replicated field plots and analysed at harvest for total N, nitrate-N and total alkaloid concentration.

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