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Laboratory evaluation of an exotoxin from Bacillus thuringiensis subsp. morrisoni to horn fly larvae (Diptera: Muscidae) and mice

Haufler, M.; Kunz, S.E.

Journal of Economic Entomology 78(3): 613-616

1985


ISSN/ISBN: 0022-0493
PMID: 4008744
DOI: 10.1093/jee/78.3.613
Accession: 001399323

Exotoxin from culture supernatant of Bacillus thuringiensis subsp. morrisoni was prepared using Sephadex G-25-40 columns and the toxin was determined by high performance liquid chromatography to be beta -exotoxin. In bioassays against larvae of Haematobia irritans, the LC50s and LC90s were estimated to be 2.79 and 8.03 micro g/g larval medium, respectively. Maximum larval mortality peaked at 98% when cattle faeces from an animal treated with 3.0 g/day for 5 days were bioassayed.

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