Penicillium multicolor beta -D-galactosidase - a comparison with Aspergillus oryzae beta -D-galactosidase
Ebata, M.; Miyake, Y.; Narita, M.; Koizumi, K.; Tsuzuki, H.; Fujiwara, T.; Morihara, K.
Saishin Igaku 39(1): 192-201
Penicillium multicolor beta -D-galactosidase was studied with a view to using it to produce low-lactose milk. It gave a single band on polyacrylamide gel disc electrophoresis at pH 4 and pH 8. Slab electrophoresis on SDS-polyacrylamide gel indicated that it was composed of a single glycoprotein unit with mol. wt. about 135 000. Its isoelectric point was pH 5.7 vs. 4.8 for Aspergillus oryzae beta -D-galactosidase.