+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Role of hormones on the induction of retinoic acid binding protein in mouse mammary gland organ culture



Role of hormones on the induction of retinoic acid binding protein in mouse mammary gland organ culture



Carcinogenesis 6(8): 1103-1107



Influence of various hormones on the induction of cellular retinoic acid binding protein (CRABP) was investigated in the mouse mammary gland organ cultures. Thoracic pairs of mammary glands from the BALB/c mice were cultured for seven days in the presence of various hormones in CMRL medium at 37 degrees C under 50% O2, 5% CO2 and 45% N2 atmosphere. There was a modest increase of mammary CRABP by insulin (I) + prolactin (P), however, addition of progesterone (Pg) or estrogen (E) + Pg to the medium resulted in a dramatic increase in the CRABP. Aldosterone (A) + hydrocortisone (F), in addition to I + P, which promotes differentiation to an extent similar to that of I + P + E + Pg did not have any influence on the induction of CRABP. These results indicate that prolactin and/or Pg in the medium can increase the concentration of CRABP in the mammary gland in vitro. From the results presented in this report, as well as previous work by other investigators, it is concluded that the biological response to retinoids in the mammary tissues cannot be correlated with the absolute concentration of CRABP in the cells. However, the biological response may be dependent upon both the ability of the target organ cells to metabolize the retinoid and to have minimal concentration of CRABP for binding to the active metabolite. The functional significance of hormone-induced CRABP is presently unknown.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001452888

Download citation: RISBibTeXText

PMID: 2990755

DOI: 10.1093/carcin/6.8.1103


Related references

The biosynthesis of spermidine as a key regulatory step in the hormonal induction of milk-protein synthesis in the organ culture of the mouse mammary gland. Biochemistry of S adenosylmethionine and related compounds: 577-580, 1982

Induction of atypical ductal hyperplasia in mouse mammary gland organ culture. Journal of the National Cancer Institute 93(14): 1103-1106, 2001

Subpopulations of cells in immature mouse mammary gland as detected by proliferative responses to hormones in organ culture. Developmental Biology 106(1): 70-75, 1984

Demethylation in the 5'-flanking region of mouse cellular retinoic acid binding protein-I gene is associated with its high level of expression in mouse embryos and facilitates its induction by retinoic acid in P19 embryonal carcinoma cells. Developmental Dynamics 201(1): 1-10, 1994

Differentiation of mammary gland during pregnancy: the response to hormones of rabbit and goat mammary gland explants in organ culture. Journal of Endocrinology 68(3): 32p-32p, 1976

Synthesis and secretion of alpha -lactalbumin by mouse mammary gland in organ culture is enhanced by the presence of thyroid hormones. Journal of Cell Biology 91(2, II): 214a, 1981

Direct channeling of retinoic acid between cellular retinoic acid-binding protein II and retinoic acid receptor sensitizes mammary carcinoma cells to retinoic acid-induced growth arrest. Molecular and Cellular Biology 22(8): 2632-2641, 2002

Binding of 7,12-dimethylbenz[a]anthracene to BALB/c mouse mammary gland DNA in organ culture. Journal of the National Cancer Institute 61(2): 465-469, 1978

Induction and prevention of carcinogen-induced precancerous lesions in mouse mammary gland organ culture. Methods in Cell Science 19(1): 19-24, 1997

Surface IgA binding by mouse mammary gland epithelial cells in culture after treatment with hormones. Federation Proceedings 39(3, II): 1153, 1980

Retinoic acid induction of mouse cellular retinoic acid-binding protein-I gene expression is enhanced by sphinganine. Molecular & Cellular Endocrinology 111(2): 207-211, 1995

Studies on the effects of hormones on the turnover of fatty acid synthetase in rabbit mammary gland in organ culture. Bio chemical journal: 148 (2) 309-320, 1975

Retinoic acid induction of mouse cellular retinoic acid-binding protein-I is enhanced by sphinganine. FASEB Journal 9(4): A835, 1995

Retinoic acid enhances cell responsiveness to epidermal growth factor in mouse mammary gland in culture. Archives of Gynecology 237(SUPPL): 240-241, 1985

Retinoic acid enhances cell responses to epidermal growth factor in mouse mammary gland in culture. Endocrinology 118(4): 1530-1536, 1986