EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectral Characterization and Proteolytic Mapping of Native 120-Kilodalton Phytochrome from Cucurbita pepo L


, : Spectral Characterization and Proteolytic Mapping of Native 120-Kilodalton Phytochrome from Cucurbita pepo L. Plant Physiology 77(4): 990-998

A spectral, immunochemical, and proteolytic characterization of native 120-kilodalton (kD) phytochrome from Cucurbita pepo L. is presented and compared with that previously reported for native 124-kD phytochrome from Avena sativa. The molecule was partially purified ( approximately 200-fold) in the phytochrome-far red-absorbing form (Pfr) in the presence of the protease inhibitor, phenylmethylsulfonyl fluoride, using a modification of the procedure initially developed to purify 124-kD Avena phytochrome. The spectral properties of the preparations obtained are indistinguishable from those described for 124-kD Avena phytochrome, including a Pfr lambda(max) at 730 nanometers, a spectral change ratio (DeltaA(r)/DeltaA(fr)) of 1.05, and negligible dark reversion of Pfr to the red-absorbing form (Pr) in the presence or absence of sodium dithionite. This lack of dark reversion in vitro contrasts with observations that Cucurbita phytochrome, like phytochrome from most other dicotyledons, exhibits substantial dark reversion in vivo. Ouchterlony double immunodiffusion analysis with polyclonal antibodies indicates that 120-kD Cucurbita phytochrome is immunologically dissimilar to 124-kD Avena phytochrome. However, despite this dissimilarity, immunoblot analyses of proteolytic digests have identified at least three spatially separate epitopes that are common to both phytochromes. Using endogeneous protease(s), a peptide map for Cucurbita phytochrome has been constructed and the role that specific domains play in the overall structure of the photoreceptor has been examined. One domain near the NH(2) terminus is critical to the spectral integrity of the molecule indicating that this domain plays a structural role analogous to that of a domain near the NH(2) terminus of Avena phytochrome. Proteolytic removal of this domain occurs preferentially in Pr and its removal shifts the Pfr lambda(max) to 722 nm, increases the spectral change ratio to 1.3, and substantially enhances the dark reversion rate. The apparent conservation of this domain among evolutionarily divergent plant species and its involvement in a conformational change upon photoconversion makes it potentially relevant to the mechanism(s) of phytochrome action. Preliminary evidence from gel filtration studies suggests that the 55-kD chromophoreless COOH-terminal region of the polypeptide contains a domain responsible for dimerization of phytochrome monomers.

(PDF same-day service: $19.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 001464795

PMID: 16664177

DOI: 10.1104/pp.77.4.990

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Chiarabaglio, P. M.; Coaloa, D., 2004: Software for evaluating poplar plantations. This article describes a method for the economic evaluating of poplar (Populus spp.) plantations. The software calculates an estimate based on forest dimensions and quality, timber prices, and harvesting costs.

Stehbens, W.E., 1966: Observations on Lankesterella hylae. Sporozoites of Lankesterella hylae were found in 12 of 13 green tree frogs (Hyla caerulea) from Gin Gin, Queensland. The intraerythrocytic sporozoites had two pale-staining vacuoles at the poles of the nucleus and frequently a dome-like protuberan...

Dhami, P. S.; Gopalakrishnan, V.; Kannan, R.; Ramanujam, A.; Salvi, Neeta; Udupa, S. R., 1998: Biosorption of radionuclides by Rhizopus arrhizus. The sorption of various radionuclides viz. 233U, 239Pu, 241Am, 144Ce, 147pM, 152+154Eu and 95Zr from aqueous nitrate medium has been studied using biomass Rhizopus arrhizus. The biosorption of 233U and 239Pu was found to be maximum at pH 6-7 where...

Costanzo, M.C.; Fox, T.D., 1995: A point mutation in the 5'-untranslated leader that affects translational activation of the mitochondrial COX3 mRNA. The 613-base 5'-untranslated leader (5'-UTL) of the Saccharomyces cerevisiae mitochondrial COX3 mRNA contains the target of an mRNA-specific translational activator complex composed of at least three nuclearly encoded proteins. We have g...

Spierings, F., 1967: Method for determining the susceptibility of trees to air pollution by artificial fumigation. An apparatus has been developed for testing the susceptibility of trees and shrubs to air pollution whilst they are growing in their natural environment. It does not disturb the prevailing climatic conditions because ambient air and the test gas a...

Becker, G., 1989: On Sinessites hispanicus Becker. Stereo-Atlas of Ostracod Shells, 161: 39-42

Rantakokko, K.; Uksila, J.; Rimpilainen, M.; Luukkainen, R.; Koulu, L.; Jansen, C.; Toivanen, P., 1996: IgA antibodies to streptococcal cell wall in psoriatic arthritis and psoriasis. Scandinavian Journal of Immunology 43(6): 718

Bataglia, O. C.; Santos, W. R. dos, 1990: Effect of the method of calculation and reference populations on diagnosis and recommendation integrated system (DRIS) indices. Results of trials comparing various methods of calculating DRIS indices in young and mature rubber (Hevea) plantations are presented and discussed.

Dimech, J.; Feniuk, W.; Humphrey, P. P. A., 1993: Antagonist effects of seglitide (MK 678) at somatostatin receptors in guinea pig isolated right atria. Somatostatin (SS) exerts a negative inotropic effect in isolated atria. Here we report that in guinea-pig isolated right atria, seglitide, a potent cyclic hexapeptide somatostatin agonist, behaves as a competitive somatostatin receptor antagonist...

Tummala, Rangarao V.; Weiss, Daniel N.; Feliciano, Zenaida; Shorofsky, Stephen R.; Gold, Michael R., 1994: The effects of defibrillation on endocardial rate-sensing leads. Circulation 90(4 PART 2): I498