EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

The membrane-anchoring systems of vertebrate acetylcholinesterase and variant surface glycoproteins of African trypanosomes share a common antigenic determinant



The membrane-anchoring systems of vertebrate acetylcholinesterase and variant surface glycoproteins of African trypanosomes share a common antigenic determinant



FEBS Letters 199(2): 182-186



Amphiphilic detergent-soluble acetylcholinesterase (AChE) from Torpedo is converted to a hydrophilic form by digestion with phospholipase C from Trypanosoma brucei or from Bacillus cereus. This lipase digestion uncovers an immunological determinant which crossreacts with a complex carbohydrate structure present in the hydrophilic form of all variant surface glycoproteins (VSG) of T. brucei. This crossreacting determinant is also detected in human erythrocyte AChE after digestion with T. brucei lipase. From these results we conclude that the glycophospholipid anchors of protozoan VSG and of AChE of the two vertebrates share common structural features, suggesting that this novel type of membrane anchor has been conserved during evolution.

(PDF emailed within 0-6 h: $19.90)

Accession: 001489493

Download citation: RISBibTeXText

PMID: 2422055

DOI: 10.1016/0014-5793(86)80476-8



Related references

The biochemistry of variant surface glycoproteins of the African trypanosomes. Biochemical Society Symposium 49: 169-181, 1984

Amino terminal sequence homology among variant surface glycoproteins of African trypanosomes. Molecular and Biochemical Parasitology 12(3): 287-298, 1984

Expression of 2 variant surface glycoproteins on individual african trypanosomes during antigen switching. Science (Washington D C) 229(4709): 190-193, 1985

Expression of two variant surface glycoproteins on individual African trypanosomes during antigen switching. Science 229(4709): 190-193, 1985

Rapid preparative scale purification of myristylated variant surface glycoproteins from African trypanosomes. Molecular and Biochemical Parasitology 17(1): 19-34, 1985

The variant surface glycoproteins of Trypanosoma equiperdum. Identification of a phosphorylated glycopeptide as the cross-reacting antigenic determinant. Febs Letters 158(1): 174-178, 1983

Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody. Infection and Immunity 41(2): 795-804, 1983

Antigenic variation in salivarian Trypanosomes--chemical characteristics of variant specific glycoproteins. Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme 26(8): 994-1013, 1981

Lyme disease spirochetes and ixodid tick spirochets share a common surface antigenic determinant defined by a monoclonal antibody Ixodes dammini, Ixodes ricinus. Infection and immunity 41(2): 795-804, 1983

Decay-acceleration factor (DAF) shares a common carbohydrate determinant with the variant surface glycoprotein (VSG) of the African Trypanosoma brucei. Journal of Immunology, 1382: 520-523, 1987

Secondary structure potential of the variant specific glycoproteins of african trypanosomes. Hoppe-Seyler's Zeitschrift fuer Physiologische Chemie 365(9): 1024, 1984

Troponin-T and glyceraldehyde-3-phosphate dehydrogenase share a common antigenic determinant. Journal of Muscle Research and Cell Motility 8(2): 118-124, 1987

Secondary structure of the variant surface glycoproteins of trypanosomes. Febs Letters 221(1): 37-42, 1987