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Vegetative propagation in vitro, by shoot proliferation, of two species of Serapias: S. olbia Verguin and S. pseudocordigera Moric. (Orchidaceae)


, : Vegetative propagation in vitro, by shoot proliferation, of two species of Serapias: S. olbia Verguin and S. pseudocordigera Moric. (Orchidaceae). Bulletin de la Societe Botanique de France, Lettres Botaniques 132(4/5): 289-300

Primary shoots, collected from tubers of both species, were cultured on a medium rich in organic substances, containing kinetin, GA3 and IAA. When they reached 5 mm in length they were transferred to a second medium containing Knudson's mineral solution, Murashige and Skoog micronutrients, vitamins B and C, sucrose and BA.

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