EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Vegetative propagation in vitro, by shoot proliferation, of two species of Serapias: S. olbia Verguin and S. pseudocordigera Moric. (Orchidaceae)






Bulletin de la Societe Botanique de France, Lettres Botaniques 132(4/5): 289-300

Vegetative propagation in vitro, by shoot proliferation, of two species of Serapias: S. olbia Verguin and S. pseudocordigera Moric. (Orchidaceae)

Primary shoots, collected from tubers of both species, were cultured on a medium rich in organic substances, containing kinetin, GA3 and IAA. When they reached 5 mm in length they were transferred to a second medium containing Knudson's mineral solution, Murashige and Skoog micronutrients, vitamins B and C, sucrose and BA.


Accession: 001507145



Related references

Ponchet M.; Beck D.; Poupet A., 1985: In vitro shoot proliferation for mass propagation of two species of serapias serapias olbia and serapias pseudocordigera orchidaceae. Two species of Serapias :S. olbia Verguin and S. pseudocordigera Moric were aseptically cultured in vitro. The primary shoots were collected on the tubers as meristems which were grown on a first medium rich in organic substances till they had rea...

Pellegrino, G.; Cafasso, D.; Widmer, A.; Soliva, M.; Musacchio A.; Cozzolino, S., 2001: Isolation and characterization of microsatellite loci from the orchid Serapias vomeracea (Orchidaceae) and cross-priming to other Serapias species. In this paper, we describe the isolation and characterization of 6 polymorphic microsatellite loci from the orchid S. vomeracea. This species is widely distributed in the Mediterranean region. Microsatellite loci were isolated from an enriched lib...

Roest, S.; Bokelmann, G.S., 1980: In vitro adventitious bud techniques for vegetative propagation and mutation breeding of potato (Solanum tuberosum L.). 1. Vegetative propagation in vitro through adventitious shoot formation. Rachis, petiole and leaflet-blade explants were either irradiated or not irradiated with X rays. Effects of X-ray dose, explant type and explant position (on the donor plant) on adventitious shoot and root formation are reported. A total of 3167 p...

Roest, S.; Bokelmann, G.S., 1981: Application of an in vitro adventitious shoot method in mutation breeding of potato. 1. Vegetative propagation by means of in vitro adventitious shoot formation. A method for producing plantlets from petiole segments is described.

Hossain M.; Ahmed G.; Hossain S.N., 1990: Rapid clonal propagation in sesbania cannabina through in vitro shoot proliferation from shoot tip explants. In vitro clonal propagation of Sesbania cannabina was achieved by culturing shoot tip explants of 7 days old seedlings on Murashige and Skoog (MS) medium supplemented with 1 mgl-1, 6-benzylaminopurine (BAP) +0.5 mgl-1 kinetin (Kn)+50 mgl-1 tyrosin...

Yanmaz, R.; Gulsen, Y.; Abak, K., 1986: In vitro propagation of cauliflower (Brassica oleraceae var. botrytis cv. Winner Osenia) by shoot-tip and curd tissue culture: influence of some auxins and BAP on shoot proliferation. In shoot tip cultures with a range of auxins + BAP [benzyladenine] at different concentrations, IBA, NAA and IAA were more effective than 2,4-D, the greatest number of regenerated shoots being obtained with 0.5 mg NAA + 2.0 mg BAP/litre. In curd c...

Mari, R.Sari, B.Ron, L.Maga, G.Usepp, P.Llegrino, F.Ancesc, B.Llusci, E.Ric, P.Rrotta, I.A Perrotta, A.D.M.Sacch, O., 2012: Comparative floral micromorphology in four sympatric species of Serapias Orchidaceae. A great variety of colours, shapes, scents, energy-rich rewards and other floral traits form an integrated system that increases pollination efficiency or reduces pollen loss. Flowers lacking energetic rewards possess a more elaborate morphology t...

Choudhary, M.L., 1991: Vegetative propagation of carnation in vitro through multiple shoot development. Shoot tips of cv. Scania Red were cultured on modified Gamborg's B5 medium consisting of 70 mg mesoinositol, 0.1 mg nicotinic acid, 2 mg glycine, 50 mg casein hydrolysate, 0.1 mg calcium pantothenate and 30 g sucrose/litre at different agar c...

Roest, S.; Bokelmann, G.S., 1981: Vegetative propagation of carnation in vitro through multiple shoot development. Nodal stem segments from the top and base of shoot cuttings of carnation cvs Scania 3-C, Calypso, Dark Orchid Beauty, Jolivette, Sam's Pride and Red Ivette previously irradiated with 30 or 60 Gray (Gy) X-rays, or untreated, were cultured in M...

Pellegrino, G.; Musacchio, A.; Noce, M.E.; Palermo, A.M.; Widmer, A., 2004: Reproductive versus floral isolation among morphologically similar Serapias L. species (Orchidaceae). Flowers of the Mediterranean orchid genus Serapias L. form small, dark tubes that vary among taxa in diameter and depth. Visiting insects use the floral tube as shelter and act as pollinators if they touch the sticky viscidium at the rear of the t...