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Viability of stored pollen grains of Malus domestica Borkh as evaluated by the incorporation of labelled nucleic acid precursors


, : Viability of stored pollen grains of Malus domestica Borkh as evaluated by the incorporation of labelled nucleic acid precursors. Caryologia 37(4): 323-330

Pollen of the M. domestica cultivars Golden Delicious and Starkrimson was stored in sulphuric acid solution in glass vials at -20 degrees C and sampled after storage for 2, 4, 8 and 12 months. Compared with that of fresh pollen, the ability to incorporate hymidine and ridine after storage decreased during storage.


Accession: 001507509

DOI: 10.1080/00087114.1984.10797710

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Related references

Bellani L.M.; Forino L.M.C.; Tagliasacchi A.M.; Sansavini S., 1984: Viability of stored pollen grains of malus domestica as evaluated by the incorporation of labeled nucleic acid precursors. Viability of stored pollen grains of M. domestica Borkh, cultivars Golden Delicious and Starkrimson, is expressed as: percentage of labeled generative and vegetative cells after feeding with 3H-thymidine and 1H-uridine; and percentage of germinate...

Bellani, L.M.; Bell, P.R., 1986: Cytoplasmic differences between the pollen grains of two cultivars of Malus domestica Borkh. correlated with viability and germination. Pollen grains of M. domestica [M. pumila] cv. Starkrimson contained well-developed mitochondria and a conspicuous endoplasmic reticulum. Pollen grains of Golden Delicious, however, contained fewer and smaller mitochondria and a less developed endo...

Tagliasacchi A.M.; Forino L.M.C.; Bellani L.M.; Avanzi S., 1985: Dynamics of pollen grain germination in 2 cultivars of malus domestica the influence of actinomycin d on fresh and stored pollen grains. The pattern of germination was detected in the presence or in the absence of actinomycin D (AmD) in fresh and stored binucleate pollen grains of 'Golden Delicious' (GD) and 'Starkrimson' (S). Pollen tube growth consisted of 2 d...

Tagliasacchi, A.M.; Forino, L.M.C.; Bellani, L.M.; Avanzi, S., 1985: Dynamics of pollen grain germination in two cultivars of Malus domestica: the influence of actinomycin D on fresh and stored pollen grains. Contrasting germination patterns, depending on the presence or absence of actinomycin D (AD) (which limited pollen tube growth) in the medium, were found in fresh and stored binucleate pollen grains of Golden Delicious (GD) and Starkrimson (S). Po...

Forino, L.M.C.; Avanzi, S., 1981: Stage heterogeneity of generative and vegetative cells in pollen grains of dehiscent anthers of two cultivars of Malus domestica Borkh: Golden Delicious and Starkrimson. Protoplasma 106(3/4): 205-210

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