+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A second mutation enhances resistance of a tobacco mutant to sulfonylurea herbicides



A second mutation enhances resistance of a tobacco mutant to sulfonylurea herbicides



Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 76(2): 177-182



Cultures of Nicotiana tabacum cells homozgous for a mutation (S4) at the SuRB locus that confers resistance to the sulfonylurea herbicides chlorsulfuron and sulfometuron methyl (Chaleff and Ray 1984; Chaleff and Bascomb 1987) were used to isolate a doubly mutant cell line (S4 Hra/S4+) resistant to even higher herbicide concentrations. Growth of cells homozygous for both the S4 and Hra mutations (S4 Hra/S4 Hra) was uninhibited by a herbicide concentration 500-fold higher than a concentration by which growth of S4+/S4+ callus was inhibited by 75%. Plants homozygous for both mutations were at least five-fold more resistant to foliar applications of chlorsulfuron than were singly mutant S4+/S4+ plants. This enhanced resistance was inherited as a single, semidominant, nuclear trait that is genetically linked to the S4 mutation. Acetolactate synthase (ALS) activity in extracts of leaves of doubly mutant (S4 Hra/S4 Hra) plants was approximately 20-fold more resistant to inhibition by chlorsulfuron and sulfometuron methyl than was ALS activity in singly mutant (S4+/ S4+) leaf extracts, which was in turn more resistant to inhibition by these compounds than was the normal enzyme. Extracts prepared from plants of these three genotypes possessed the same ALS specific activities. Therefore, Hra represents a second independent mutation at or near the SuRB locus that reduces the sensitivity of tobacco ALS activity to inhibition by sulfonylurea herbicides.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001518858

Download citation: RISBibTeXText

PMID: 24232103

DOI: 10.1007/bf00257843


Related references

Transformation with a mutant arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides. Molecular & General Genetics 211(2): 266-271, 1988

Semidominant soybean mutation for resistance to sulfonylurea herbicides. Crop Science 29(6): 1403-1408, 1989

Genetic manipulations of tobacco and soybean for resistance to sulfonylurea herbicides. Abstracts of papers, 192nd ACS National Meeting: AGRO 3, 1986

Cross-resistance to short residual sulfonylurea herbicides in transgenic tobacco plants. Plant Physiology 91(2): 574-580, 1989

Resistance to the sulfonylurea herbicides chlorsulfuron amidosulfuron and dpx r9674 in transgenic flue cured tobacco. Crop Science 32(4): 1049-1053, 1992

Molecular characterization of Als1, an acetohydroxyacid synthase mutation conferring resistance to sulfonylurea herbicides in soybean. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 126(12): 2957-2968, 2013

Cross-resistance pattern and alternative herbicides for Cyperus difformis resistant to sulfonylurea herbicides in Korea. Pest Management Science 60(1): 85-94, 2004

Characterization of a spontaneous rapeseed mutant tolerant to sulfonylurea and imidazolinone herbicides. Plant Breeding 111(2): 132-141, 1993

Reversal of clavulanate resistance conferred by a Ser-244 mutant of TEM-1 beta-lactamase as a result of a second mutation (Arg to Ser at position 164) that enhances activity against ceftazidime. Antimicrobial Agents and ChemoTherapy 38(5): 1134-1139, 1994

Identification of resistance to imazapyr and cross resistance to selected sulfonylurea herbicides in Kochia scoparia. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz (Sonderh. 17): 383-388, 2000

Resistance in weeds to sulfonylurea herbicides. British Crop Protection Council Brighton Crop Protection Conference: Weeds, Vols 1, 2 And 3; International Conference, Brighton, England, Uk, November 20-23, Xxii+408p (Vol 1); Xxi+394p (Vol 2); Xxii+435p (Vol 3) British Crop Protection Council: Surrey, England, Uk Illus Paper 295-300, 1989

Resistance to sulfonylurea herbicides in chickweed. Tidsskrift for Planteavls Specialserie ( S 2178): 147-156, 1992

Molecular biology of resistance to sulfonylurea herbicides. ACS Symposium Series ( 379): 25-36, 1989

Expression of a rice CYP81A6 gene confers tolerance to bentazon and sulfonylurea herbicides in both Arabidopsis and tobacco. Plant Cell, Tissue and Organ Culture 109(3): 419-428, 2012