+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Architecture of a plant promoter: S1 nuclease hypersensitive features of maize Adh1



Architecture of a plant promoter: S1 nuclease hypersensitive features of maize Adh1



Plant Molecular Biology 8(4): 299-307



S1 nuclease was used to probe the architectural characteristics of the maize alcohol dehydrogenase-1 gene promoter. Three sites were identified as hypersensitive to S1 digestion in supercoiled, but not linear plasmids containing the Adh1 gene. The sites mapped to areas located 65, 330 and 800 base pairs 5' to the start of transcription. In each case, the strand specific nicking pattern was determined with nucleotide level precision. The -65 site was found to be a homopurine/homopyrimidine tract. The -330 site mapped to the boundaries of a region of high Z-DNA potential and the -800 site mapped to a non-descript sequence. The possible biological significance of these sites is discussed.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001532097

Download citation: RISBibTeXText

PMID: 24301192

DOI: 10.1007/bf00021309


Related references

Site-specific oligodeoxynucleotide binding to maize Adh1 gene promoter represses Adh1-GUS gene expression in vivo. Plant molecular biology: an international journal on molecular biology biochemistry and genetic engineering 19(5): 715-723, 1992

Constitutive and anaerobically induced DNase-I-hypersensitive sites in the 5' region of the maize Adh1 gene. Proceedings of the National Academy of Sciences of the United States of America 84(3): 799-803, 1987

The maize Adh1 promoter : structures in supercoiled DNA. Maize Genetics Cooperation Newsletter (60): 62, 1986

The maize Adh1 promoter: chromatin structure. Maize Genetics Cooperation Newsletter (60): 61-62, 1986

Chemical detection of Z-DNA within the maize Adh1 promoter. Plant molecular biology: an international journal on molecular biology biochemistry and genetic engineering 18(6): 1181-1184, 1992

Characterization of the G-quadruplexes in the duplex nuclease hypersensitive element of the PDGF-A promoter and modulation of PDGF-A promoter activity by TMPyP4. Nucleic Acids Research 35(22): 7698-7713, 2007

Thermodynamics of i-tetraplex formation in the nuclease hypersensitive element of human c-myc promoter. Biochemical and Biophysical Research Communications 320(4): 1220-1227, 2004

A nuclease-hypersensitive element of the human c-myc promoter interacts with a transcription initiation factor. Molecular and Cellular Biology 9(11): 5123-5133, 1989

A nuclease hypersensitive element in the human c-myc promoter adopts several distinct i-tetraplex structures. Biochemical and Biophysical Research Communications 278(1): 158-166, 2000

Effect of the promoter and the first intron of maize Adh1 on foreign gene expression in rice. Maydica 35(4): 353-357, 1990

Osmium tetroxide footprinting of a scaffold attachment region in the maize Adh1 promoter. Plant Molecular Biology 22(6): 1145-1151, 1993

The TATA box promoter region of maize Adh1 affects its organ-specific expression. Embo Journal 11(1): 157-166, 1992

PuF/NM23-H2/NDPK-B transactivates a human c-myc promoter-CAT gene via a functional nuclease hypersensitive element. Oncogene 10(12): 2343-2347, 1995