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Assignment of genes encoding dihydrofolate reductase and hexosaminidase B to Mus musculus chromosome 13



Assignment of genes encoding dihydrofolate reductase and hexosaminidase B to Mus musculus chromosome 13



Somatic Cell and Molecular Genetics 12(6): 641-648



The gene encoding dihydrofolate reductase (DHFR) was studied by complementation mapping. Microcells prepared from diploid mouse fibroblasts were fused with mutant hamster cells lacking the dihydrofolate reductase gene (Dhfr), and DHFR+ microcell hybrids were selected in medium lacking purines and pyrimidines. The complemented hybrids expressed wild-type levels of DHFR activity, and selectively retained a single mouse chromosome (No. 13).

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