+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Attenuation of chicken anaemia agent by serial passages in cell cultures

Attenuation of chicken anaemia agent by serial passages in cell cultures

Journal of Veterinary Medicine, B Infectious Diseases, Immunology, Food Hygiene, Veterinary Public Health 33(8): 568-573

Chicken anaemia agent (CAA) was attenuated by serial passages in cultures of the MDCC-MSB 1 cell line. Infectivity of CAA was assayed in cell cultures (TCID50) and in day-old chickens (chicken-ID50). Pathogenicity of CAA was significantly decreased after only 12 passages in cell culture; it decreased further, but was not completely lost during an additional 100 passages. Titres of virulent CAA propagated in chickens were, on average, lower by 1.4 log in chickens than in cell culture.

(PDF emailed within 1 workday: $29.90)

Accession: 001533698

Download citation: RISBibTeXText

Related references

Attenuation of chicken anemia agent (CAA) by serial passages in cell culture. Journal of veterinary medicine: Series B = Zentralblatt fur Veterinarmedizin: Reihe B 33(8): 568-573, 1986

Serial propagation and purification of chicken anaemia agent in MDCC-MSB1 cell line. Avian Pathology 16(1): 149-163, 1987

Attenuation of the agent of avian infectious anemia (CAA) by serial passage in cell cultures. Zentralblatt für Veterinarmedizin. Reihe B. Journal of Veterinary Medicine. Series B 33(8): 568-573, 1986

Chicken anaemia agent in Egypt: I. A serological survey of antibody against chicken anaemia agent in some commercial chicken flocks using indirect immunofluorescent technique. Veterinary Medical Journal Giza 42(3): 53-58, 1994

Study of the genetic stability of measles virus CAM-70 vaccine strain after serial passages in chicken embryo fibroblasts primary cultures. Virus Genes 36(1): 35-44, 2007

II. Serial passages and growth curves in various cell cultures. Jap. J. vet. Sci, Chemo-sero-therapeutic Res. Inst., Kuma-moto., 27: 171-177, 1965

Effect of multiple cell culture passages on the biological behaviour of chicken anaemia virus. Avian Pathology 27(1): 74-79, 2008

Studies on bovine enteroviruses. II. Serial passages and growth curves in various cell cultures. Nihon Juigaku Zasshi. Japanese Journal of Veterinary Science 27(3): 171-178, 1965

Antigenicity and pathogenicity characteristics of molecularly cloned chicken anaemia virus isolates obtained after multiple cell culture passages. Archives of Virology 144(10): 1961-1975, 1999

Virulence-associated genome mutations of murine rotavirus identified by alternating serial passages in mice and cell cultures. Journal of Virology 88(10): 5543-5558, 2014

Genetic stability of the attachment glycoprotein of human respiratory syncytial viruses during serial passages in cell cultures. Acta Virologica 48(2): 115-121, 2004

Stability of the glycoprotein gene of avian metapneumovirus (Canada goose isolate 15a/01) after serial passages in cell cultures. Avian Diseases 54(2): 915-918, 2010

Peculiar genetic characteristics of a mutant of the sanarelli virus obtained by passages in cell cultures abstract rabbit attenuation. Presse Medicale 76(8): 398, 1968

Clinical findings in chicken infectious anaemia induced by chicken runting agent (CRA) and the physicochemical characteristics of this agent. Bulletin of the Nippon Veterinary and Zootechnical College (33): 49-53, 1984