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Attenuation of chicken anaemia agent by serial passages in cell cultures



Attenuation of chicken anaemia agent by serial passages in cell cultures



Journal of Veterinary Medicine, B Infectious Diseases, Immunology, Food Hygiene, Veterinary Public Health 33(8): 568-573



Chicken anaemia agent (CAA) was attenuated by serial passages in cultures of the MDCC-MSB 1 cell line. Infectivity of CAA was assayed in cell cultures (TCID50) and in day-old chickens (chicken-ID50). Pathogenicity of CAA was significantly decreased after only 12 passages in cell culture; it decreased further, but was not completely lost during an additional 100 passages. Titres of virulent CAA propagated in chickens were, on average, lower by 1.4 log in chickens than in cell culture.

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Accession: 001533698

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