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Australian standard diagnostic techniques for animal diseases. No. 45. Measurement of blood glutathione peroxidase activity for assessment of selenium nutrition in livestock



Australian standard diagnostic techniques for animal diseases. No. 45. Measurement of blood glutathione peroxidase activity for assessment of selenium nutrition in livestock



Australian standard diagnostic techniques for animal diseases No 45 Measurement of blood glutathione peroxidase activity for assessment of selenium nutrition in livestock: 13



Activity of glutathione peroxidase in erythrocytes is quantitatively determined by coupling its reaction to NADPH-dependent glutathione reductase. Hydrogen peroxide is used as the peroxide substrate, and a linear reaction rate is observed at 340 nM. Activity of the enzyme in blood is expressed relative to the haemoglobin concentration. Activities of glutathione peroxidase associated with adequate and inadequate selenium nutrition in a number of domestic species are tabulated.

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