+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Autofluorescence of bovine ligamentum nuchae, cartilage, heart valve and lung measured by microscopy and fibre optics



Autofluorescence of bovine ligamentum nuchae, cartilage, heart valve and lung measured by microscopy and fibre optics



Research in Veterinary Science 45(2): 230-233



The fluorescence of bovine tissues was measured post mortem by microscopy of frozen sections and by using optical fibres to excite fluorescence and to measure fluorescence emission spectra. Mechanical disruption of the tissue (by comminution or sectioning) did not apparently change tissue fluorescence spectra. Ligamentum nuchae had the strongest fluorescence and lung tissue had the weakest. In samples measured with a minimum prior exposure to ultraviolet light, the peak fluorescence emission was at 410or 420 nm (with excitation at 365 nm). Exposure to ultraviolet light for about 1 minute shifted the fluorescence peak to 450 to 470 nm. Further exposure (about 30 minutes) caused a loss of the 450 to 470 nm fluorescence peak, while emissions above 530 nm were maintained or strengthened. Microscopy showed that the fluorescence that was measured by fibre optics from intact connective tissues originated mostly from collagen and elastin fibres.

(PDF emailed within 1 workday: $29.90)

Accession: 001533913

Download citation: RISBibTeXText

PMID: 3194593



Related references

Autofluorescence of adipose tissue measured with fibre optics. Meat Science 19(4): 277-284, 1987

Identification of putative novel protein from bovine ligamentum nuchae and human lung elastin. American Review of Respiratory Disease 137(4 PART 2): 176, 1988

The preparation procedure of crosslinking amino acids containing quaternary pyridinium skeleton from bovine ligamentum nuchae aorta and lung. Animal Science & Technology 62(2): 186-193, 1991

Studies on lysyl oxidase of bovine ligamentum nuchae and bovine aorta. Advances in Experimental Medicine and Biology 79: 531-542, 1977

Ruptured bovine ligamentum nuchae. Jour Amer Vet Med Assoc 127(942): 214, 1955

Comparative studies of the cross-linked regions of elastin from bovine ligamentum nuchae and bovine, porcine and human aorta. Biochemical Journal 149(3): 685-695, 1975

The swelling of bovine ligamentum nuchae as a function of pH. Biochemical Journal 96(3): 813-817, 1965

Guanidination of kappa-elastin from bovine ligamentum nuchae. Paroi Arterielle 5(2): 69-74, 1979

Dielectric relaxation studies on bovine ligamentum nuchae. Biopolymers 27(11): 1787-1793, 1988

Characterisation of the microfibrillar component of bovine ligamentum nuchae. Biochemical and Biophysical Research Communications 65(3): 1146-1152, 1975

Studies on the chemistry of elastin bovine ligamentum nuchae. Brookhaven Symposia in Biology 21: 358-377, 1968