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Automated micromethod for determining 3-hydroxybutyrate in bovine plasma without acid precipitation



Automated micromethod for determining 3-hydroxybutyrate in bovine plasma without acid precipitation



Annales de Recherches Veterinaires 17(2): 129-139



An automated micro-method for the determination of 3-hydroxybutyrate (3-HB) in plasma samples which were not deproteinised was compared with a manual method using deproteinised samples. The methods were based on the production of QNADH by the reaction of 3-HB with NAD catalysed by 3-HB dehydrogenase. Computer analysis in the automated method allowed 3-HB levels to be measured quickly and at low cost. Aceto-acetate and acetone levels did not interfere with 3-HB determination.

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Accession: 001533954

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