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Beet cryptic virus - purification and serological detection in four varieties of Beta vulgaris L






Archiv fur Phytopathologie und Pflanzenschutz 23(2): 95-104

Beet cryptic virus - purification and serological detection in four varieties of Beta vulgaris L

A protocol for partial purification of BCrV from beet leaves is given. Two light-scattering zones were formed in sucrose density gradients of purified preparations. TEM showed these zones to contain morphologically different isometric particles. The max. virus yield was calculated to be 1 mg/kg leaves. Preparations containing both types of particles were used to produce antisera. Titres against BCrV ranged from 1:4096 to 1:16 384.


Accession: 001535389



Related references

Kuehne T.; Stanarius A.; Plobner L.; Schubert J., 1988: Occurrence of beet cryptic virus 1 and beet cryptic virus 2 in single plants of sugar beet and in other varieties of beta vulgaris l. The occurrence of beet cryptic virus 1 (BCV-1) and beet cryptic virus 2 (BCV-2) was investigated in fodder beet (cv. Rote Walze), red beet (cv. Rote Kugel) and spinach beet (cv. Lukullus). For the purpose, the BCV complex was purified was from lea...

Kuhne, T.; Stanarius, A.; Plobner, L.; Schubert, J., 1988: On the occurrence of beet cryptic virus 1 and beet cryptic virus 2 in single plants of sugarbeet and in other varieties of Beta vulgaris L. The occurrence of BCV-I and BCV-II was investigated in cultivars Rote Walze (fodder beet), Rote Kugel (beetroot) and Lukullus (spinach beet). The BCV complex was purified from leaf material of the 3 cultivars, and the positions of the virus zones...

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Xie, W.S.; Antoniw, J.F.; White, R.F.; Woods, R.D., 1993: A third cryptic virus in beet (Beta vulgaris). Japanese leaf beet (Beta vulgaris var. cicla [spinach beet]) cv. Fundanso plants contained 4 double-stranded RNA (dsRNA) components in apparently healthy beet plants. Two were identified as originating from beet cryptic I alphacryptovirus (BCIV),...

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