Combined electron-spin-resonance, X-ray-diffraction studies on phospholipid vesicles obtained from cold-hardened wheats : I. An attempt to correlate electron-spin-resonance spectral characteristics with frost resistance

Vigh, L.; Horváth, I.; Woltjes, J.; Farkas, T.; van Hassett, P.; Kuiper, P.J.

Planta 170(1): 14-19


ISSN/ISBN: 0032-0935
PMID: 24232836
DOI: 10.2307/23378911
Accession: 001548924

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Phospholipid multibilayers, obtained from thermally acclimatized Triticum aestivum cv. Penjamo 62 (P62), sensitive to frost, and cv. Mironovskaya 808 (M808), frost resistant, were investigated. Electron-spin-resonance (ESR) revealed 2 breaks in the motion of the spin-labelled fatty acid 2-(14-carboxytetradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl, for both cultivars (+3 degrees C, -17 degrees C for M808 and +5 degrees C, -18 degrees C for P62) when grown at 22 degrees C. M808 compensated for exposure to cold (+2 degrees C) by shifting the onset of the apparent phase-separation temperature from +3 degrees C to -16 degrees C. P62 was unable to do so. It is suggested that the development of frost tolerance in wheat may be related to the ability to prevent the formation of "ordered lipid domains" as discernible by ESR.